Nuclear antisense RNA. An efficient new method to inhibit gene expression.
Nuclear antisense RNA. An efficient new method to inhibit gene expression.
复制标题
核反义RNA。
DOI：
10.1007/bf02824803
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发表时间：
1994
影响因子：
2.6
通讯作者：
Carmichael,GG
中科院分区：
文献类型：
--
作者：
Liu,Z;Carmichael,GG
我们描述了一种有效的新的反义RNA方法来抑制基因表达。保留在细胞核中的反义RNA与靶转录物结合，并似乎导致其靶的降解。如果反义RNA的3′端不被多聚腺苷酸化，它们可以特异性地在细胞核中表达和积累。在反义表达载体中，我们使用顺式作用核酶产生3′-末端，而不依赖于多聚腺苷酸化机制，从而抑制RNA分子从细胞核转运到细胞质。我们在小鼠多瘤病毒模型系统中评估了这种方法，其中病毒早期转录区的核反义转录本有效地降低了病毒早链RNA的水平。非特异性反义RNA对病毒基因表达无影响。在比较研究中，核反义RNA在下调多瘤病毒早期RNA方面比常规反义分子显著更有效，所述常规反义分子通过聚腺苷酸化处理。
We describe an efficient new antisense RNA method to inhibit gene expression. Antisense RNAs that are retained in the nucleus bind to target transcripts and appear to lead to the degradation of their targets. Antisense RNAs can be expressed and accumulated specifically in the nucleus if they are not polyadenylated at their 3′ ends. In antisense expression vectors we use a cis-acting ribozyme to generate 3′-ends independently of the polyadenylation machinery and thereby inhibit transport of RNA molecules from the nucleus to the cytoplasm. We have evaluated this method in the mouse polyoma virus model system, where nuclear antisense transcripts to the viral early transcription region efficiently reduced the level of viral early-strand RNAs. Nonspecific antisense RNA had no effects on viral gene expression. In comparative studies, nuclear antisense RNAs were significantly more effective in downregulating polyoma virus early RNAs than were conventional antisense molecules, which were processed by polyadenylation.