5-15-DIHETE AND LIPOXINS GENERATED BY NEUTROPHILS FROM ENDOGENOUS ARACHIDONIC-ACID AS ASTHMA BIOMARKERS
5-15-DIHETE AND LIPOXINS GENERATED BY NEUTROPHILS FROM ENDOGENOUS ARACHIDONIC-ACID AS ASTHMA BIOMARKERS
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DOI：
10.1006/bbrc.1995.1183
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发表时间：
1995-02-06
影响因子：
3.1
通讯作者：
GODARD, P
中科院分区：
文献类型：
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作者：
CHAVIS, C;CHANEZ, P;GODARD, P
5-本文研究了在离子载体A23187的体外刺激下，健康人（HS）和哮喘患者（AP）的人血多形核细胞（PMN）产生脂氧素、5，15-二羟基二十碳四烯酸（5，15-diHETE）和白三烯的能力。中性粒细胞从AP能够产生更高的白三烯水平从内源性来源比中性粒细胞从HS。此外，它们以线性方式产生5，15-diHETE（从50到280 ng/ 10（7）细胞）和脂氧素（从1到30 ng/ 10（7）细胞），而在相同的实验条件下，HS的PMN中没有检测到这些化合物的量。血液PMN的增强的5-LO活化可能反映了跨细胞信号传导引发，表明脂氧素和5，15-diHETE可能是比白三烯B-4更特异的炎症状态生物标志物。(C)出版社：Academic Press
5-Lipoxygenase (5-LO) activation of human blood polymorphonuclear cells (PMN) from healthy subjects (HS) and from asthmatic patients (AP) was investigated comparing their respective capacities to produce lipoxins, 5,15-dihydroxyeicosatetraenoic acid (5,15-diHETE) and leukotrienes, under in vitro stimulation by ionophore A23187. PMN from AP were able to generate higher leukotriene levels from endogenous sources than PMN from HS. Moreover they produced 5,15-diHETE (from 50 to 280ng / 10(7) cells) and lipoxins (from 1 to 30ng / 10(7) cells), in a linear manner, whereas in the same experimental conditions no detectable amounts of these compounds appeared in PMN from HS. The enhanced 5-LO activation of blood PMN may reflect transcellular signalisation priming indicating that lipoxins and 5,15-diHETE could be much more specific inflammatory state biomarkers than leukotriene B-4. (C) 1995 Academic Press, Inc.