A structural basis for the difference in specificity between the two jacalin-related lectins from mulberry (Morus nigra) bark
A structural basis for the difference in specificity between the two jacalin-related lectins from mulberry (Morus nigra) bark
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DOI：
10.1016/s0006-291x(03)00538-2
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发表时间：
2003-04-25
影响因子：
3.1
通讯作者：
Van Damme, EJM
中科院分区：
文献类型：
--
作者：
Rougé, P;Peumans, WJ;Van Damme, EJM
半乳糖特异性和甘露糖特异性jacalin相关的凝集素从黑桑葚（桑）树的树皮的活性和特异性已重新调查使用不同的实验方法。这两种凝集素肯定表现为多特异性凝集素识别半乳糖，甘露糖，葡萄糖，即使MornigaG和MornigaM相互作用优先与半乳糖和甘露糖，分别。这两种凝集素的碳水化合物结合位点的异常扩展的大小显然占其多特异性。与其他甘露糖特异性jacalin相关凝集素的平行研究证实，它们对甘露糖/葡萄糖的特异性依赖于其碳水化合物结合位点的尺寸减小。(C)2003 Elsevier Science（美国）。All rights reserved.
The activity and specificity of a galactose-specific and a mannose-specific jacalin-related lectin from the bark of the black mulberry (Morus nigra) tree has been re-investigated using different experimental approaches. Both lectins definitely behave as polyspecific lectins recognizing galactose, mannose, and glucose even though MornigaG and MornigaM interact preferentially with galactose and mannose, respectively. The exceptionally extended size of the carbohydrate-binding site of both lectins apparently accounts for their polyspecific character. Parallel studies with other mannose-specific jacalin-related lectins confirmed that their exclusive specificity towards mannose/glucose relies on a reduced size of their carbohydrate-binding site. (C) 2003 Elsevier Science (USA). All rights reserved.