Diethylstilbestrol increases the density of prolactin cells in male mouse pituitary by inducing proliferation of prolactin cells and transdifferentiation of gonadotropic cells
Diethylstilbestrol increases the density of prolactin cells in male mouse pituitary by inducing proliferation of prolactin cells and transdifferentiation of gonadotropic cells
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DOI：
10.1007/s00418-005-0141-6
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发表时间：
2006-07-01
影响因子：
2.3
通讯作者：
Koji, Takehiko
中科院分区：
文献类型：
--
作者：
Shukuwa, Keiko;Izumi, Shin-ichi;Koji, Takehiko
己烯雌酚（DES）与哺乳动物异常有关。研究了不同剂量DES对雄性小鼠垂体促卵泡激素（FSH）、促黄体生成素（LH）和催乳素（PRL）细胞的影响。DES降低FSH和LH细胞的密度呈剂量依赖性，但增加PRL细胞的密度。当评估雌激素受体(ER) α和β的表达时，发现DES主要在PRL细胞中诱导ER β。然而，由于这些作用在ER α敲除小鼠中被消除，DES似乎主要通过ER α起作用。在DES处理后的不同时间点检测PRL细胞中Ki-67和Pit-1的表达，部分PRL细胞在10-15天呈Ki-67阳性，在5-15天呈Pit-1阳性细胞增多。此外，一些FSH和LH细胞在5-10天时变为Pit-1阳性，并与PRL共定位。我们的研究结果表明，DES通过诱导PRL细胞增殖和FSH/LH细胞向PRL细胞的转分化来增加PRL细胞。
Diethylstilbestrol (DES) has been implicated in mammalian abnormalities. We examined the effects of DES on follicle-stimulating hormone (FSH), luteinizing hormone (LH), and prolactin (PRL) cells in the pituitaries of male mice treated with various doses of DES for 20 days. DES reduced the density of FSH and LH cells in a dose-dependent manner, but increased that of PRL cells. When the expression of estrogen receptor (ER) alpha and beta was assessed, an induction of ER beta by DES was found predominantly in PRL cells. However, since these effects were abolished in ER alpha knockout mice, DES appears to act primarily through ER alpha. When the expression of Ki-67 and Pit-1 in PRL cells was examined at various time-points after DES treatment, some PRL cells became Ki-67 positive at 10-15 days, and Pit-1-positive cells were increased at 5-15 days. Furthermore, some FSH and LH cells became Pit-1 positive, and co-localized with PRL at 5-10 days. Our results indicate that DES increases PRL cells by inducing proliferation of PRL cells and transdifferentiation of FSH/LH cells to PRL cells.