Characterization of a Mus spretus YAC that maps to the pseudoautosomal region.
Characterization of a Mus spretus YAC that maps to the pseudoautosomal region.
复制标题
映射到假常染色体区域的 Mus spretus YAC 的表征。
DOI：
10.1006/geno.1996.4462
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Elliott,RW
中科院分区：
文献类型：
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作者：
Yen,CH;Elliott,RW
一个库，包含M.制备半YAC载体中的spretusDNA并筛选与端粒六聚体TTAGGG的寡聚体杂交的克隆。脾细胞中期扩散的 FISH 显示克隆 YTY3 与 X 和 Y 染色体远端杂交，这与伪常染色体区域 (PAR) 的定位一致。使用质粒亚克隆和 YTY3 的 PCR 引物对 BXD RI 菌株和种间回交进行重组作图，显示与远端 Chr X 的连锁。YAC 的限制性图谱包含三个 NotI 位点。与Mov15类似的序列靠近克隆的载体末端，而另一端包含似乎位于PAR内的端粒阵列，表明插入片段代表PAR的近端部分。与克隆同源的序列也存在于常染色体 4、9 和 13 的亚端粒区域。
A library, containingM. spretusDNA in a half-YAC vector, was made and screened for clones hybridizing with an oligomer of the telomere hexamer TTAGGG. FISH to metaphase spreads of spleen cells showed hybridization of clone YTY3 to the distal ends of both X and Y chromosomes, consistent with localization to the pseudoautosomal region (PAR). Recombinational mapping in the BXD RI strains and an interspecies backcross, using a plasmid subclone and PCR primers from YTY3, showed linkage to distal Chr X. The restriction map of the YAC contains threeNotI sites. Sequences similar toMov15lie close to the vector end of the clone, while the other end contains a telomere array that appears to be interstitial within the PAR, suggesting that the insert represents a proximal portion of the PAR. Sequences homologous to the clone are also present at subtelomeric regions of autosomes 4, 9, and 13.