Expression of vascular endothelial growth factor A, B, C, and D in oral squamous cell carcinoma
Expression of vascular endothelial growth factor A, B, C, and D in oral squamous cell carcinoma
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DOI：
10.1016/s1368-8375(03)00127-1
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发表时间：
2004-01-01
期刊：
影响因子：
4.8
通讯作者：
Hamakawa, H
中科院分区：
文献类型：
--
作者：
Shintani, S;Li, CN;Hamakawa, H
血管内皮生长因子（VEGF）A在肿瘤血管生成中起重要作用。已经发现了VEGF家族的其他成员VEGF B、C和D。VEGF C和D对淋巴管内皮细胞显示一定的选择性。然而，VEGF家族成员是否在肿瘤血管生成和淋巴结转移中发挥作用在很大程度上尚不清楚。本研究旨在探讨VEGF家族成员在口腔鳞状细胞癌（OSCC）中的作用。我们通过免疫组化、逆转录-聚合酶链反应和蛋白质印迹法评估VEGF家族成员的表达。所有VEGF成员在口腔鳞癌中均有不同程度的表达。VEGF家族成员表达和微血管密度的免疫组化分析显示VEGF A和B表达与肿瘤血管生成相关。尽管VEGF A和B在淋巴结阳性和淋巴结阴性的口腔鳞癌中均有表达，但与淋巴结阴性的肿瘤相比，VEGF C和D在淋巴结阳性的肿瘤中的表达更频繁。这些发现表明VEGF C和/或D的表达水平与淋巴肿瘤扩散的发展之间可能存在关系。(C)2003 Elsevier Ltd.保留所有权利。
Vascular endothelial growth factor (VEGF) A is known to play an important role in tumor angiogenesis. The additional members of the VEGF family, VEGF B, C and D have been discovered. VEGF C and D show some selectivity toward lymphatic endothelial cells. However, whether VEGF family members play a role in tumor angiogenesis and lymph node metastasis is largely unknown. The aim of the present study was to explore the role of VEGF family members in oral squamous cell carcinoma (OSCC). We evaluated the expression of VEGF family members by immunohistochemistry, reverse transcription-polymerase chain reaction, and western blotting. All VEGF members were expressed at different levels in OSCC. Immunohistological analysis of VEGF family members expression and microvessel density revealed a correlation between VEGF A and B expression and tumor angiogenesis. Although VEGF A and B expression were detected in both node-positive and node-negative OSCC, VEGF C and D expression was detected frequently in node-positive tumors compared to node-negative tumors. These findings suggest a possible relationship between the expression level of VEGF C and/or D and development of lymphatic tumor spread. (C) 2003 Elsevier Ltd. All rights reserved.