Identification of central nervous system sites involved in the water diuresis response elicited by central microinjection of nociceptin/ Orphanin FQ in conscious rats via c-Fos and inducible cAMP early repressor immunocytochemistry.
Identification of central nervous system sites involved in the water diuresis response elicited by central microinjection of nociceptin/ Orphanin FQ in conscious rats via c-Fos and inducible cAMP early repressor immunocytochemistry.
复制标题
通过 c-Fos 和诱导型 cAMP 早期阻遏物免疫细胞化学，在清醒大鼠中中央显微注射痛敏肽/孤啡宁 FQ 来鉴定参与水利尿反应的中枢神经系统部位。
DOI：
10.1111/j.1365-2826.2007.01559.x
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发表时间：
2007
影响因子：
3.2
通讯作者：
Cunningham,JT
中科院分区：
文献类型：
--
作者：
Gottlieb,HB;Fleming,TM;Ji,L;Cunningham,JT
Intracerebroventricular (i.c.v.) administration of the opioid‐like peptide, nociceptin/Orphanin (nociceptin), in conscious rats produces diuretic and antinatriuretic effects. The present study utilised changes in Fos and inducible cAMP early repressor (ICER) immunocytochemistry expression to examine the central nervous (CNS) sites activated or inhibited, respectively, by central administration of nociceptin. Urine samples were collected during control (15 min) and after i.c.v. vehicle (5 µl, n = 12) or nociceptin (10 µg/5 µl; n = 12). Four additional urine samples (15‐min) were collected after the i.c.v. injection. The brain was processed for Fos using a commercially available antibody (Oncogene AB‐5) and for ICER using a polyclonal anti‐ICER antibody raised in rabbits. In vehicle‐injected conscious rats, renal excretion of water or sodium was not altered. However, nociceptin produced a rapid and marked increase in urine flow (V) and a decrease in urinary sodium excretion rate. In addition, i.c.v. nociceptin produced a significant increase in Fos staining in the dorsomedial nucleus of the hypothalamus, the perinuclear zone of the supraoptic nucleus, the organum vasculosum of the lamina terminalis (OVLT), the lateral preoptic area and the lateral hypothalamic area compared to control. By contrast, Fos expression decreased in the area postrema and locus coeruleus compared to controls. Furthermore, ICER staining was significantly increased in the perinuclear zone of the supraoptic nucleus, supraoptic nucleus, median preoptic nucleus, OVLT, medial preoptic area, central nucleus of the amygdala, and medial nucleus of the solitary tract. Together, central opioid receptor‐like type 1 activation in these CNS regions may participate in the neural pathways involved in the diuretic and antinatriuretic effects of nociceptin.
