Indirect measurement of nitric oxide production by monitoring nitrate and nitrite using microchip electrophoresis with electrochemical detection
Indirect measurement of nitric oxide production by monitoring nitrate and nitrite using microchip electrophoresis with electrochemical detection
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DOI：
10.1021/ac0204000
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发表时间：
2002-12-15
影响因子：
7.4
通讯作者：
Lunte, SM
中科院分区：
文献类型：
--
作者：
Kikura-Hanajiri, R;Martin, RS;Lunte, SM
建立了微芯片毛细管电泳电化学检测一氧化氮（NO）的方法。该方法结合了测定亚硝酸盐的直接安培检测的微芯片为基础的CE分离和转化硝酸盐为亚硝酸盐的化学还原使用铜涂层镉颗粒。通过计算硝酸盐还原前后样品中亚硝酸盐的量的差异来定量硝酸盐的量。的分离，注射，检测和还原反应条件的优化，以及涉及集成到微芯片上的还原反应的研究，进行了描述。结果表明，微芯片CEEC可在45 s内分离检测亚硝酸根。还原反应成功地集成在芯片上，并在激活Cd颗粒后约1分钟内进行。该装置的实用性被证明通过监测从3-morpholinosydnonimine，NO释放化合物产生的硝酸盐和亚硝酸盐的量。
An indirect method for monitoring nitric oxide (NO) by determining nitrate and nitrite using microchip capillary electrophoresis (CE) with electrochemical (EC) detection has been developed. This method combines determination of nitrite by direct amperometric detection following a microchip-based CE separation and conversion of nitrate to nitrite by chemical reduction using Cu-coated Cd granules. The amount of nitrate is quantified by calculating the difference in the amount of nitrite in the sample before and after the reduction of nitrate. Optimization of the separation, injection, detection, and reduction reaction conditions, as well as studies involving integration of the reduction reaction onto the microchip, are described. It was found that nitrite can be separated and detected in similar to45 s by microchip CEEC. The reduction reaction was successfully integrated on-chip and carried out in similar to1 min following activation of the Cd granules. The usefulness of this device was demonstrated by monitoring the amount of nitrate and nitrite produced from 3-morpholinosydnonimine, a NO-releasing compound.