Effects of ovarian ischemia-reperfusion on luteal function in pregnant rats.
Effects of ovarian ischemia-reperfusion on luteal function in pregnant rats.
复制标题
卵巢缺血再灌注对妊娠大鼠黄体功能的影响。
作者：
Norihiro Sugino;Yasuhiko Nakamura;N. Okuno;Masaya Ishimatu;T. Teyama;H. Kato
据报道，活性氧参与 CL 回归。我们已经证明，怀孕第 15 天后，血清孕酮浓度的下降与超氧化物歧化酶 (SOD) 活性的下降和 CL 中脂质过氧化物水平的增加同时发生。最近发现缺血再灌注会刺激活性氧的产生并导致各种器官的组织损伤。因此，我们测试了妊娠大鼠卵巢缺血再灌注对 CL 功能的影响。妊娠第15天，夹紧双侧卵巢血管30分钟后，松开卵巢再灌注90分钟。再灌注90分钟后，缺血再灌注降低了CL中的血清孕酮浓度和SOD活性，并增加了CL中的脂质过氧化物。同时注射SOD和过氧化氢酶可以完全阻断缺血再灌注的影响，包括血清孕酮浓度的降低，但不能通过给予吲哚美辛来阻断。本研究表明，CL 功能受到卵巢缺血再灌注产生的活性氧的抑制，并且该作用不是通过前列腺素介导的。
An involvement of reactive oxygen species in CL regression has been reported. We have shown that a decrease in serum progesterone concentrations coincides with a decrease in superoxide dismutase (SOD) activities and an increase in lipid peroxide levels in the CL after Day 15 of pregnancy. Recently it has been found that ischemia-reperfusion stimulates reactive oxygen species production and causes tissue damage in various organs. We therefore tested the effect of ischemia-reperfusion in the ovary on CL function in pregnant rats. On Day 15 of pregnancy, after clamping of the bilateral ovarian vessels for 30 min, the ovaries were reperfused for 90 min by declamping. The ischemia-reperfusion decreased serum progesterone concentration and SOD activity in the CL and increased lipid peroxide in the CL 90 min after reperfusion. The effects of ischemia-reperfusion, including the decrease in serum progesterone concentrations, were completely blocked by simultaneous injection of SOD and catalase, but not by indomethacin administration. The present study shows that CL function was inhibited by reactive oxygen species produced by ischemia-reperfusion in the ovary and that the effect was not mediated through prostaglandins.
登录
查看更多内容
影响因子：
--
作者：
HERNANDEZ, LA;GRISHAM, MB;GRANGER, DN
通讯作者：
GRANGER, DN
影响因子：
4.8
作者：
BEHRMAN, HR;ATEN, RF
通讯作者：
ATEN, RF
影响因子：
--
作者：
GRISHAM, MB;HERNANDEZ, LA;GRANGER, DN
通讯作者：
GRANGER, DN
DOI：
10.1152/ajpendo.1991.260.2.e170
发表时间：
1991-02
期刊：
The American journal of physiology
影响因子：
--
作者：
N. Tanaka;L. Espey;T. Kawano;H. Okamura
通讯作者：
N. Tanaka;L. Espey;T. Kawano;H. Okamura
整体缺血后线粒体过氧化氢的产生以及谷胱甘肽过氧化物酶和超氧化物歧化酶的活性。
影响因子：
5
作者：
Shlafer,M;Myers,CL;Adkins,S
通讯作者：
Adkins,S