Apical junction complex protein expression in the canine colon: Differential expression of claudin-2 in the colonic mucosa in dogs with idiopathic colitis
Apical junction complex protein expression in the canine colon: Differential expression of claudin-2 in the colonic mucosa in dogs with idiopathic colitis
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DOI：
10.1369/jhc.7a7211.2007
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发表时间：
2007-10-01
影响因子：
3.2
通讯作者：
Miller, Hugh R. P.
中科院分区：
文献类型：
--
作者：
Ridyard, Alison E.;Brown, Jeremy K.;Miller, Hugh R. P.
犬特发性淋巴细胞-浆细胞性结肠炎（LPC）是犬中公认的临床和病理实体，与免疫细胞群和细胞因子表达谱的改变相关。临床和实验数据表明，肠上皮细胞通透性的改变有助于一系列相关疾病的发病机制。顶端连接复合体在调节上皮细胞旁通透性方面起着重要作用，我们已经表征了其组成部分紧密连接（ZO-1，occludin，claudin-2）和粘附连接（E-钙粘蛋白和β-连环蛋白）蛋白在正常结肠和特发性LPC犬结肠中的分布。ZO-1、闭合蛋白、E-钙粘蛋白和β-连环蛋白在正常犬结肠中的分布与先前在人类和啮齿动物中描述的相似。与人类的情况相反，在正常犬结肠隐窝上皮中观察到claudin-2特异性标记，从远端到近端隐窝的强度降低，并且在结肠的腔表面几乎检测不到。在特发性LPC影响的犬中，ZO-1、occludin、E-cadherin或β-catenin表达的显著变化的证据很少。然而，claudin-2的表达显着增加，在受影响的狗的近端隐窝和腔结肠上皮，这表明在犬LPC的发病机制中的作用。
Canine idiopathic lymphocytic-plasmacytic colitis (LPC) is a well-recognized clinical and pathological entity in the dog, associated with altered immune cell populations and cytokine expression profiles. Clinical and experimental data indicate that alterations in the permeability of the intestinal epithelium contribute to the pathogenesis of a range of related conditions. The apical junction complex plays a significant role in regulating epithelial paracellular permeability, and we have characterized the distribution of a number of its component tight junction (ZO-1, occludin, claudin-2) and adherens junction (E-cadherin and beta-catenin) proteins in normal colon and colon from dogs with idiopathic LPC. ZO-1, occludin, E-cadherin, and beta-catenin exhibited a distribution in normal canine colon similar to that described previously in humans and rodents. In contrast to the situation in humans, claudin-2specific labeling was observed in the normal canine colonic crypt epithelium, decreasing in intensity from the distal to the proximal crypt and becoming barely detectable at the luminal surface of the colon. There was little evidence for significant changes in ZO-1, occludin, E-cadherin, or beta-catenin expression in dogs affected by idiopathic LPC. However, claudin-2 expression markedly increased in the proximal crypt and luminal colonic epithelium in affected dogs, suggesting a role in the pathogenesis of canine LPC.