Genome-Wide Analysis of Whole Human Glycoside Hydrolases by Data-Driven Analysis in Silico
Genome-Wide Analysis of Whole Human Glycoside Hydrolases by Data-Driven Analysis in Silico
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DOI：
10.3390/ijms20246290
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发表时间：
2019-12-01
影响因子：
5.6
通讯作者：
Ito, Masahiro
中科院分区：
文献类型：
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作者：
Nakamura, Takahiro;Fahmi, Muhamad;Ito, Masahiro
葡聚糖通过糖基转移酶和糖苷水解酶参与多种代谢过程。分析这些酶的进化对于提高对葡聚糖代谢和功能的理解至关重要。基于我们先前对糖基转移酶的研究，我们使用UniProt、Brenda、CAZy和KEGG数据库对整个人类糖苷水解酶进行了全基因组分析。根据与脊索动物或后生动物(1类)、后生动物(2类)、后生动物和植物(3类)和真核生物(4类)中保守的酶的相似性，将319个人糖苷水解酶分为4类。真核生物和后生动物分别含有N-糖苷水解酶和O-糖苷水解酶。在最保守的簇中有大量的无序区域，这表明无序区域在糖苷水解酶的进化中发挥了作用。这些结果表明，多细胞生物的生物多样性与N-和O-连接的葡聚糖的获得有关。
Glycans are involved in various metabolic processes via the functions of glycosyltransferases and glycoside hydrolases. Analysing the evolution of these enzymes is essential for improving the understanding of glycan metabolism and function. Based on our previous study of glycosyltransferases, we performed a genome-wide analysis of whole human glycoside hydrolases using the UniProt, BRENDA, CAZy and KEGG databases. Using cluster analysis, 319 human glycoside hydrolases were classified into four clusters based on their similarity to enzymes conserved in chordates or metazoans (Class 1), metazoans (Class 2), metazoans and plants (Class 3) and eukaryotes (Class 4). The eukaryote and metazoan clusters included N- and O-glycoside hydrolases, respectively. The significant abundance of disordered regions within the most conserved cluster indicated a role for disordered regions in the evolution of glycoside hydrolases. These results suggest that the biological diversity of multicellular organisms is related to the acquisition of N- and O-linked glycans.