Triple-Bioinspired Burying/Crosslinking Interfacial Coassembly Strategy for Layer-by-Layer Construction of Robust Functional Bioceramic Self-Coatings for Osteointegration Applications
Triple-Bioinspired Burying/Crosslinking Interfacial Coassembly Strategy for Layer-by-Layer Construction of Robust Functional Bioceramic Self-Coatings for Osteointegration Applications
复制标题
用于骨整合应用的稳健功能生物陶瓷自涂层的逐层构建的三重仿生埋入/交联界面共组装策略
DOI：
10.1021/acsami.8b20429
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发表时间：
2019-01-30
影响因子：
9.5
通讯作者：
Zheng, Yufeng
中科院分区：
文献类型：
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作者：
Jia, Zhaojun;Xiu, Peng;Zheng, Yufeng
将具有固有生物活性的生物陶瓷涂层在生物金属植入物上是解决后者骨整合不良的一种直接而有希望的解决方案。更进一步，开发一种温和、廉价、通用的方法来稳定任何陶瓷在任何植入基板上的原理，同时赋予预期的多功能生物功能性能，将是一项非同一般的成就。在此，我们描述了一种三重生物激发的掩埋/交联界面协同组装策略，用于实现这种陶瓷涂层，该策略巧妙地融合了来自海洋岩石（掩埋辅助颗粒固定化）、海洋贻贝（普遍粘附和通用化学反应性）和造礁牡蛎（交联呈现凝聚力）的生物激发。具体来说，表面功能化的水性分散陶瓷颗粒被埋在基质锚定的聚电解质多层有机基质中（即聚醚亚胺（PEI)/聚对苯乙烯磺酸钠（PSS））），通过一种新的无机有机杂化层接层（LBL）共组装方案，其中贻贝（牡蛎）激发的粘合剂（内聚）化学被精心编排。作为概念演示，我们合成了具有生物活性的巴格达迪石（Ca3ZrSi2O9）作为模型陶瓷，并利用上述策略构建了医用钛坚固，仿生和可交联的LBL自组装体。衬底接触紧密，埋藏良好的无机有机界面明显，具有良好的结构和化学稳定性，特别是交联后。体外证实了持续的生物活性离子释放和明显的生物矿化活性。随后，系统地研究了这些组装体的生物学性能，包括表面亲水性、蛋白质吸附和成骨细胞功能。此外，纳米银沉积赋予表面额外的抗菌能力，被用来证明该策略的多功能性。更重要的是，通过修改临床相关的复杂3D多孔支架，证明了我们方法的灵活性。总体而言，我们的策略基本达到了设计预期，预示着未来的医疗应用前景良好。该研究为骨植入物的生物活性和功能性表面设计提供了一种广泛有用的替代途径。它也可能为生物和其他应用的其他多生物启发材料/界面提供见解。
Coating bioceramics of inherent bioactivity onto biometallic implants is a straightforward yet promising solution to address poor osteointegration of the latter. One step further, it would be a nontrivial accomplishment to develop a mild, cheap, and universal route to firmly stabilizing, in principle, any ceramics onto any implant substrate, while imparting expectedly versatile biofunctional performances. Herein, we describe a triple-bioinspired burying/cross-linking interfacial coassembly strategy for enabling such ceramic coatings, which ingeniously fuses bioinspiration from sea rocks (burying assisted particle immobilization), marine mussels (universal adhesion and versatile chemical reactivity), and reef-building oysters (cross-linking rendered cohesion). Specifically, surface functionalized, aqueous dispersed ceramic particles were buried within an substrate-anchored organic matrix of polyelectrolyte multilayers (i.e., (poly(ether imide) (PEI)/poly(sodium-p-styrenesulfonate) (PSS))), through a new inorganic organic hybrid layer-by-layer (LBL) coassembly scheme wherein mussel (oyster) inspired adhesive (cohesive) chemistries were exquisitely orchestrated. As a conceptual demonstration, bioactive baghdadite (Ca3ZrSi2O9) was synthesized as model ceramics, with which we constructed on medical titanium robust, biomimetic, and cross-linkable LBL self-assemblies harnessing the said strategy. Intimate substrate contacts and well-defined buried inorganic organic interfaces were evidently seen, together with good structural and chemical stabilities, especially after cross-linking. Sustained bioactive ion releasing and appreciable biomineralization activity were confirmed in vitro. Subsequently, biological performances of the assemblies were systematically investigated with respect to surface hydrophilicity, protein adsorption, and osteoblast functions. Additionally, nanosilver deposition, which imparted the surfaces with added antibacterial potencies, was used to exemplify the strategy's versatility in allowing multifunctionality. What's more, the flexibility of our approach was testified through modifying clinically relevant complicated 3D porous scaffolds. Overall, our strategy basically met the design expectations, boding well for future medical adoption. This study offers the promise of an alternative broadly useful avenue to bioactive and functional surface design of bone implants. It may also provide insights into other multiple-bioinspired materials/interfaces for biological and other applications.