Yos1p is a novel subunit of the Yip1p-Yif1p complex and is required for transport between the endoplasmic reticulum and the golgi complex
Yos1p is a novel subunit of the Yip1p-Yif1p complex and is required for transport between the endoplasmic reticulum and the golgi complex
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DOI：
10.1091/mbc.e04-10-0873
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发表时间：
2005-04-01
影响因子：
3.3
通讯作者：
Barlowe, C
中科院分区：
文献类型：
--
作者：
Heidtman, M;Chen, CZ;Barlowe, C
酵母Yip 1 p是与Rab GTP酶相互作用的保守跨膜蛋白家族的成员。以前的研究也表明了Yip 1 p在内质网（ER）衍生的COPII转运囊泡的生物发生中的作用。在这份报告中，我们描述了鉴定和表征的开放阅读框架YER 074 W-A作为一种新的多拷贝抑制剂的热敏yip 1 -4菌株。我们将该基因命名为Yip One Suppressor 1（YOS 1）。Yos 1 p对生长和分泌途径的功能至关重要; Yos 1 p的缺失或失活阻断ER和高尔基体复合体之间的转运。YOS 1编码一个87个氨基酸的膜蛋白，在真核生物中是保守的。Yos 1 p定位于ER和高尔基体膜，并被有效地包装到ER衍生的COPII转运囊泡中。Yos 1 p与Yip 1 p和Yif 1 p结合，表明Yos 1 p是Yip 1 p-Yif 1 p复合物的一个新亚基。
Yeast Yip1p is a member of a conserved family of transmembrane proteins that interact with Rab GTPases. Previous studies also have indicated a role for Yip1p in the biogenesis of endoplasmic reticulum (ER)-derived COPII transport vesicles. In this report, we describe the identification and characterization of the uncharacterized open reading frame YER074W-A as a novel multicopy suppressor of the thermosensitive yip1-4 strain. We have termed this gene Yip One Suppressor 1 (YOS1). Yos1p is essential for growth and for function of the secretory pathway; depletion or inactivation of Yos1p blocks transport between the ER and the Golgi complex. YOS1 encodes an integral membrane protein of 87 amino acids that is conserved in eukaryotes. Yos1p localizes to ER and Golgi membranes and is efficiently packaged into ER-derived COPII transport vesicles. Yos1p associates with Yip1p and Yif1p, indicating Yos1p is a novel subunit of the Yip1p-Yif1p complex.