A PIEZOELECTRIC IMMUNOSENSOR FOR THE DETECTION OF CORTISOL
A PIEZOELECTRIC IMMUNOSENSOR FOR THE DETECTION OF CORTISOL
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DOI：
10.1080/00032719508000035
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发表时间：
1995-01-01
影响因子：
2
通讯作者：
SULEIMAN, AA
中科院分区：
文献类型：
--
作者：
ATTILI, BS;SULEIMAN, AA
研制了一种用于皮质醇检测的压电晶体免疫传感器。将皮质醇抗体层压在10 MHz压电晶体的金电极上，该压电晶体预涂有蛋白A或戊二醛。用蛋白A预包被的晶体在稳定性和灵敏度方面显示出最好的结果。该传感器被成功地用于测定皮质醇的标准溶液从36-3628 μ g/L（十亿分之一）。该传感器的优点包括简单，分析时间短，成本效益和选择性。结果表明，皮质醇测定在临床检验和药物监测中是可行的。
A piezoelectric crystal immunosensor has been developed for the detection and determination of cortisol. Cortisol antibody was layered onto the gold electrodes of a 10 MHz piezoelectric crystal which was precoated with either protein A or gluteraldehyde. Crystals pre-coated with protein A showed the best results with respect to stability and sensitivity. The sensor was successfully used for the determination of cortisol in standard solutions from 36-3628 mu g/L (part per billion). The advantages of the proposed sensor include simplicity, short analysis time, cost effectiveness and selectivity. The results demonstrate the feasibility of cortisol assay in clinical testing and in drug monitoring.