A diguanylate cyclase regulates biofilm formation in Rhodococcus sp. NJ-530 from Antarctica
A diguanylate cyclase regulates biofilm formation in Rhodococcus sp. NJ-530 from Antarctica
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DOI：
10.1007/s13205-021-03093-z
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发表时间：
2021-12
期刊：
影响因子：
2.8
通讯作者：
Xixi Wang;Yingying He;Yashan Deng;Zhi Zuo;Dan Li;Fushan Chen;Chang-feng Qu;J. Miao
中科院分区：
文献类型：
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作者：
Xixi Wang;Yingying He;Yashan Deng;Zhi Zuo;Dan Li;Fushan Chen;Chang-feng Qu;J. Miao
生物膜代表了一种保护性的生存模式，其中细菌为了生存目的而使自己适应自然环境。生物膜的形成受3，5-环二鸟苷酸（c-di-GMP）的调节，其是细菌中的通用第二信使分子。二鸟苷酸环化酶（DGC）催化细胞内c-di-GMP的合成，在细菌适应自然环境中发挥重要作用。本研究首次从舌红球菌中克隆了DGC基因。NJ-530. DGC基因全长948个核苷酸，编码315个氨基酸，分子量为34.6 KDa，等电点为5.58。qRT-PCR结果表明，DGC的表达水平受低盐度和低温度的影响。同样的胁迫条件下，生物膜的形成率较高。结果表明，红球菌属Rhodococcussp. NJ-530能够适应南极的极端环境，这与生物膜的形成密切相关。这些结果为研究南极微生物对这种极端环境的适应机制提供了重要参考。
Biofilms represent a protective survival mode in which bacteria adapt themselves to the natural environment for survival purposes. Biofilm formation is regulated by 3,5-cyclic diguanylic acid (c-di-GMP), which is a universal second messenger molecule in bacteria. Diguanylate cyclase (DGC) catalyses c-di-GMP intracellular synthesis, which plays important roles in bacterial adaptation to the natural environment. In this study, theDGCgene was first cloned from AntarcticRhodococcussp. NJ-530.DGCcontained 948 nucleotides and encoded 315 amino acids with a molecular weight of 34.6 KDa and an isoelectric point of 5.58. qRT–PCR demonstrated that theDGCexpression level was significantly affected by lower salinity and temperature. Consistently, more biofilm formation occurred under the same stress. It has been shown thatRhodococcussp. NJ-530 can adapt to the extreme environment in Antarctica, which is closely related to biofilm formation. These results provide an important reference for studying the adaptive mechanism of Antarctic microorganisms to this extreme environment.