Intraocular antibody synthesis against rubella virus and other microorganisms in Fuchs' heterochromic cyclitis
Intraocular antibody synthesis against rubella virus and other microorganisms in Fuchs' heterochromic cyclitis
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DOI：
10.1007/s00417-009-1239-7
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发表时间：
2010-04-01
影响因子：
2.7
通讯作者：
Pleyer, Uwe
中科院分区：
文献类型：
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作者：
Ruokonen, Peter C.;Metzner, Sylvia;Pleyer, Uwe
Fuchs异色性睫状体炎（FHC）是一种常见的眼内疾病，病因不明，最近与风疹病毒（RV）感染有关。我们用63例连续FHC患者的房水和血清样本研究了病毒特异性IgG的合成。此外，还测定了抗单纯疱疹病毒（HSV）、水痘带状疱疹病毒（VZV）、巨细胞病毒（CMV）和刚地弓形虫的眼内免疫球蛋白G的产生。在20例患者中，对E1基因进行RT-PCR检测RV RNA。46例HSV和vzv相关性葡萄膜炎、HLA B-27阳性前葡萄膜炎和Posner-Schlossman综合征患者作为对照。在所有63例FHC患者中证实了针对RV的特异性眼内抗体合成，而46例对照中没有一例RV IgG阳性。有趣的是，在11例RV IgG合成阳性的患者中，可以检测到额外的HSV (8)， VZV(1)和CMV(2)特异性抗体。在FHC患者中，只有两次用PCR检测到病毒RNA。在这一系列报道的最大的FHC患者中，我们检测到FHC与眼内抗风疹病毒抗体合成之间的强烈关联。此外，在11例患者中，有可能确认额外的眼内抗体合成，特别是HSV。房水中pcr阳性结果仅为RV。与其他研究相比，RV基因组仅在两名患者（10%）中被鉴定出来。
Fuchs' heterochromic cyclitis (FHC) is a common intraocular disease of uncertain etiology that has recently been related to rubella virus (RV) infection.We investigated the synthesis of RV-specific IgG using aqueous humor and serum samples from 63 consecutive patients with FHC. In addition, intraocular immunoglobulin G production against herpes simplex virus (HSV), varicella zoster virus (VZV), cytomegalovirus (CMV) and toxoplasma gondii was determined. In 20 patients, the detection of RV RNA was additionally performed by RT-PCR on the E1 gene. Forty-six patients with HSV- and VZV-associated uveitis, HLA B-27 positive anterior uveitis, and Posner-Schlossman syndrome served as controls.Specific intraocular antibody synthesis against RV was confirmed in all 63 FHC patients, whereas none of the 46 controls was positive for RV IgG. Interestingly, in 11 patients with positive RV IgG synthesis, additional HSV (eight), VZV (one) and CMV (two) specific antibodies could be detected. Only twice was viral RNA detectable by PCR in a patient with FHC.In this largest reported series of FHC patients, we detected a strong association between FHC and intraocular antibody synthesis against rubella virus. Furthermore, in 11 patients, it was possible to confirm an additional intraocular antibody synthesis, in particular HSV. PCR-positive results in the aqueous humor were exclusively obtained for RV. In contrast to other studies, the RV genome could only be identified in two patients (10%).