Identification of rat bone morphogenetic protein-3b (BMP-3b), a new member of BMP-3
Identification of rat bone morphogenetic protein-3b (BMP-3b), a new member of BMP-3
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DOI：
10.1006/bbrc.1996.0289
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发表时间：
1996-02-15
影响因子：
3.1
通讯作者：
Kangawa, K
中科院分区：
文献类型：
--
作者：
Takao, M;Hino, J;Kangawa, K
以人骨形态发生蛋白-3（BMP-3）的简并寡核苷酸为引物，通过RT-PCR方法从大鼠股骨cDNA文库中分离得到BMP-3和BMP-3b的cDNA。推导的大鼠BMP-3成熟区氨基酸序列与人BMP-3相比有2个氨基酸变化（98%的同一性）。推导的BMP-3b氨基酸序列与大鼠BMP-3成熟区的相似性为81%，与前肽区的相似性仅为37%。北方印迹法检测大鼠肋骨、肋骨、股骨、颅骨、气管、主动脉和脑中BMP-3 b mRNA的表达。在这些组织中，BMP-3b转录本主要在小脑中表达。BMP-3 mRNA在股骨、颅骨、气管、肺和卵巢中均有表达。虽然BMP-3b和BMP-3彼此非常密切相关，但它们的转录物分布在不同的组织中，除了两者都在骨中发现。BMP-Sb mRNA的分布模式表明，BMP-3b在中枢神经系统以及骨形成和重建中起重要作用。(C)出版社：Academic Press，Inc.
Rat bone morphogenetic protein-3 (BMP-3) cDNA and BMP-3b cDNA were isolated from rat femur cDNA library by the RT-PCR method using degenerate oligonucleotide primers corresponding to human BMP-3. The deduced amino acid sequence of rat BMP-3 in the mature region reveals 2 amino acid changes compared to that of human BMP-3 (98% identity). The deduced BMP-3b amino acid sequence shows 81% similarity with the mature region of rat BMP-3 and only 37% similarity with the propeptide region. By Northern blot analysis, rat BMP-3b mRNA was detected in costa, costicartilage, femur, calvaria, trachea, aorta and brain. Among these tissues, BMP-3b transcripts were predominantly expressed in cerebellum. BMP-3 mRNA was found in femur, calvaria, trachea, lung and ovary. Although BMP-3b and BMP-3 are very closely related to each other, their transcripts are distributed in different tissues except that both are found in bone. The distribution pattern of BMP-Sb mRNA suggests that BMP-3b plays an important role in the central nervous system as well as in bone formation and remodeling. (C) 1996 Academic Press, Inc.