Ascaris: Development of selected genotypes in mice
Ascaris: Development of selected genotypes in mice
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蛔虫：小鼠选定基因型的发育
DOI：
10.1016/j.exppara.2012.03.006
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发表时间：
2012-05-01
影响因子：
2.1
通讯作者：
Hu, Ningyan
中科院分区：
文献类型：
--
作者：
Peng, Weidong;Yuan, Keng;Hu, Ningyan
利用核糖体DNA第一内转录间隔区的核苷酸变异，已鉴定出五种不同的基因类型(命名为G1-G5)，人类的G1型和猪的G3型的优势导致了携带这两种基因的寄生虫对特定宿主物种的亲和力。随后利用来自人类的G1型卵子和来自猪的G3型卵子感染猪和小鼠的研究表明，这两种基因在感染小鼠和猪作为幼虫和成年猪的能力方面存在显著差异。在前人研究的基础上，本研究调查了已知基因型蛔虫菌株在发育方面是否存在差异，如卵孵化、幼虫迁移和在小鼠体内分布。用G1型和G3型蛔虫卵经口感染C57BL/6小鼠。感染后每隔2小时从小肠、大肠、胸腔、腹腔、外周血、肝脏和肺中取卵/幼虫进行检查，直至感染34天。结果表明，两个品系在卵孵化(孵化时间、孵化位置和孵化数量)、幼虫迁移和分布(幼虫的方式和构成比、恢复高峰的时间和幼虫在肠道中的重现)方面存在明显差异。这一结果可以解释小鼠体内G3型蛔虫幼虫回收率显著高于G3型幼虫的现象，并对了解不同基因型蛔虫宿主从属关系的差异具有一定的启示作用。(C)2012 Elsevier Inc.保留所有权利。
Using nucleotide variation in the first internal transcribed spacer of nuclear ribosomal DNA, five different genotypes (designated G1-G5) have been identified and the preponderance of genotype G1 in humans and of genotype G3 in pigs led to the proposal that parasites bearing the two genotypes have an affinity for a particular host species. A subsequent study using eggs of genotype G1 from humans and G3 from pigs to infect pigs and mice indicated that there is a significant difference in the ability to infect and establish as larvae in mice and as adults in pigs between the two genotypes. Extending previous investigations, the present study investigated whether there are differences in development as designated by egg hatching, larvae migration and distribution in the mice between the Ascaris strains with known genotypes. Ascaris eggs of genotypes G1 (predominating in human-derived worms) and G3 (predominating in pig-derived worms) were used to infect C57BL/6 mice orally. Eggs/larvae were examined from the small and large intestines, thoracic and abdominal cavities, peripheral blood, livers and lungs at intervals of 2 h until 12 h post-infection, then periodically until 34 days of infection. Results showed distinct differences in egg hatching (the timing and location of hatching, and the numbers hatched), and in larvae migration and distribution (the means and constituent ratios, the time of peak recovery, and larvae reappearing in intestines) between the two strains. The results can explain the findings of significantly higher larval recovery of genotype G1 than G3 in the mice, and may shed some enlightenment to understand the difference in host affiliation of Ascaris of different genotypes. (C) 2012 Elsevier Inc. All rights reserved.