Development-dependent expression of complex-type sugar chains specific to mouse brain.
Development-dependent expression of complex-type sugar chains specific to mouse brain.
复制标题
小鼠大脑特有的复杂型糖链的发育依赖性表达。
DOI：
10.1093/oxfordjournals.jbchem.a022056
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发表时间：
1998
影响因子：
2.7
通讯作者：
S. Hase
中科院分区：
文献类型：
--
作者：
S. Nakakita;S. Natsuka;K. Ikenaka;S. Hase
我们先前检测到具有二等分GlcNAc残基的岩藻糖基半乳糖双天线（BA-2）和缺乏与BA-2的Manalpha 1 -3残基连接的GlcNAc残基的岩藻糖基半乳糖双天线（BA-1），它们在小鼠脑中特异性富集[Shimizu，H.，Ochiai，K.，Ikenaka，K.，Mikoshiba，K.，和Hase，S. 114，334-338]。从不同年龄的小鼠脑中制备吡啶氨基糖链，并通过HPLC分离后定量BA-1和BA-2。在大脑中，BA-1在新生小鼠大脑中几乎不表达，但在发育过程中表达量逐渐增加，而BA-2在出生后1周表达量达到最大值，随后迅速下降;在成年小鼠中，BA-1和BA-2的表达量几乎相同。小脑中BA-1的表达在各期均低于BA-2。具有BA-1和BA-2结构的糖蛋白富集在膜组分中，并且溶解的糖蛋白通过凝集素亲和层析和凝胶过滤纯化。结果表明，BA-1和BA-2在小脑中存在于大于20 kDa的糖蛋白中，而在大脑中则主要存在于80-200 kDa的糖蛋白中。这些结果表明，这两个脑特异性糖链发育调节和连接到亚细胞器的膜相关糖蛋白。
We previously detected a fucosylagalactobiantenna with a bisecting GlcNAc residue (BA-2) and one lacking the GlcNAc residue linked to the Manalpha1-3 residue of BA-2 (BA-1), which were enriched specifically in mouse brain [Shimizu, H., Ochiai, K., Ikenaka, K., Mikoshiba, K., and Hase, S. (1993) J. Biochem. 114, 334-338]. Pyridylamino sugar chains were prepared from mouse brains of various ages, and BA-1 and BA-2 were quantified after separation by HPLC. In cerebrum, BA-1 was scarcely expressed in newborn brain but gradually increased in amount during development, while expression of BA-2 reached a maximum 1 week after birth followed by a rapid decrease; in adult mice, the amount of BA-1 was almost the same as that of BA-2. In cerebellum, expression of BA-1 was lower than that of BA-2 at all stages. Glycoproteins with the BA-1 and BA-2 structures were enriched in the membrane fraction, and the glycoproteins solubilized were purified by lectin-affinity chromatography and gel filtration. The results indicated that BA-1 and BA-2 occurred in glycoproteins of more than 20 kDa in cerebellum, but most BA-1 and BA-2 were found in a 80-200 kDa fraction in cerebrum. These results show that the two brain-specific sugar chains are developmentally regulated and linked to the membrane-associated glycoproteins of subcellular organellas.