Facial nerve identification with fluorescent dye in rats
Facial nerve identification with fluorescent dye in rats
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DOI：
10.1590/s0102-865020160020000003
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发表时间：
2016-02-01
影响因子：
1.1
通讯作者：
Abrahao, Marcio
中科院分区：
文献类型：
--
作者：
Melo, Giulianno Molina de;Cervantes, Onivaldo;Abrahao, Marcio
目的：腮腺切除术技术仍然有较高的轻瘫和瘫痪指数，降低患者的生活质量。正确识别面神经可以防止神经损伤。荧光染料在实验研究中识别神经，但只有少数文献将其用于腮腺切除术中的面神经研究。方法：成年雄性Wistar大鼠40只，面部注射生理盐水（GSF对照组，10只）或荧光染料溶液（Gdye组，30只），然后行保留面神经的腮腺切除术，测量定位时间和定位易度（LocTime和LFN）。结果：Gdye组神经定位快，Easy LFN率为83%，与对照组比较差异有统计学意义（P < 0.05）。Gdye组神经损伤程度较轻，PMV和PFP评分较好，敏感性和准确性较高。结论：面神经荧光实验方法可有效用于术中神经的可视化、识别和保存。该技术可用于未来的面神经研究，转化为人类，有助于在不久的将来优化腮腺手术。
PURPOSE: The parotidectomy technique still has an elevated paresis and paralysis index, lowering patient life's quality. The correct identification of the facial nerve can prevent nerve damage. Fluorescent dye identifies nerves in experimental studies but only few articles focused its use on facial nerve study in parotidectomies. We aimed to stain the rat facial nerve with fluorescent dye to facilitate visualization and dissection in order to prevent injuries.METHODS: Forty adult male Wistar rats were submitted to facial injection of saline solution (Gsf-control group, 10) or fluorescent dye solution (Gdye group, 30) followed by parotidectomy preserving the facial nerve, measuring the time for localization and facility of localization (LocTime and LFN). Nerve function was assessed using the Vibrissae Movements (PMV) and Eyelid Closure Motion (PFP) scores.RESULTS: Nerve localization was faster in Gdye group, with 83% Easy LFN rate. The Gdye group presented with low nerve injury degree and better PMV and PFP scores, with high sensitivity and accuracy.CONCLUSIONS: This experimental method of facial nerve fluorescence was effective for intraoperative nerve visualization, identification and preservation. The technique may be used in future facial nerve studies, translated to humans, contributing to the optimization of parotid surgery in the near future.