NEW CD44 SPLICE VARIANTS ASSOCIATED WITH HUMAN BREAST CANCERS
NEW CD44 SPLICE VARIANTS ASSOCIATED WITH HUMAN BREAST CANCERS
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DOI：
10.1002/jcp.1041620115
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发表时间：
1995-01-01
影响因子：
5.6
通讯作者：
BOURGUIGNON, LYW
中科院分区：
文献类型：
--
作者：
IIDA, N;BOURGUIGNON, LYW
细胞表面CD 44变体（CD 44 v）亚型的变化与肿瘤转移相关。在这项研究中，我们已经研究了CD 44变异亚型的表达在人类乳腺癌样本的各种技术，包括免疫组化，逆转录-聚合酶链反应（RT-PCR），核苷酸测序。使用RT-PCR，我们已经确定，正常人乳腺组织主要含有CD 44上皮（CD 44 E）形式和非常少的CD 44标准（CD 44 s）形式。然而，转移性乳腺癌似乎过表达CD 44 E和CD 44 s形式，并显示多种新的CD 44变体同种型。使用抗CD 44抗体（识别糖蛋白CD 44类的共同决定簇）的组织细胞化学染色证实了CD 44分子过表达并优先位于转移性乳腺癌组织中。核苷酸测序分析表明，至少有四种新的CD 44变体同种型（即，通过外显子7、10、11和14的插入或缺失显示独特剪接）可能与人转移性乳腺癌密切相关。这些新描述的CD 44变体亚型可能有助于监测人类乳腺癌转移的进展。(C)1995年Wiley-Liss，Inc.
Changes in the CD44 variant (CD44v) isoforms on the cell surface have been correlated with tumor metastasis. In this study we have examined the expression of CD44 variant isoforms in human breast carcinoma samples by a variety of techniques including immunohistochemistry, reverse transcriptase-polymerase chain reaction (RT-PCR), and nucleotide sequencing. Using RT-PCR, we have determined that normal human breast tissue contains primarily the CD44 epithelial (CD44E) form and very little CD44 standard (CD44s) form. However, metastatic breast carcinomas appear to overexpress both the CD44E and CD44s forms and also display multiple new species of CD44 variant isoforms. Histocytochemical staining using anti-CD44 antibody (recognizing a com mon determinant of the CD44 class of glycoproteins) confirms that the CD44 molecules are overexpressed and preferentially located in metastatic breast cancer tissues. Nucleotide sequencing analyses indicate that at least four new CD44 variant isoforms (i.e., displaying unique splicing via the insertion or the deletion of exons 7, 10, 11, and 14) may be closely associated with human metastatic breast cancers. These newly described CD44 variant isoforms may be useful for monitoring the progression of human breast cancer metastasis. (C) 1995 Wiley-Liss, Inc.