Annexin-1 localization in human skin: possible association with cytoskeletal elements in keratinocytes of the stratum spinosum.
Annexin-1 localization in human skin: possible association with cytoskeletal elements in keratinocytes of the stratum spinosum.
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DOI：
10.1111/1523-1747.ep12371684
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发表时间：
1993-11
期刊：
影响因子：
--
通讯作者：
R. Fava;L. Nanney;D. Wilson;L. King
中科院分区：
文献类型：
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作者：
R. Fava;L. Nanney;D. Wilson;L. King
膜联蛋白-1（也称为脂皮质素-1或p35），表皮生长因子/受体激酶、蛋白激酶C和转氨酶的假定底物，在胚胎、新生儿、成人和患病的人表皮中进行免疫定位。在胚胎皮肤强烈的膜联蛋白-1免疫反应被发现在peridendum在54天估计胎龄（EGA）。后来（EGA = 91-143天），膜联蛋白-1免疫反应仅限于基底角质形成细胞。在新生儿皮肤中，基底细胞往往比基底上角质形成细胞染色更重，这也被染色。只有基底角质形成细胞染色在成人足底皮肤，但在薄的皮肤膜联蛋白-1存在于基底，基底上，有时甚至在表皮的颗粒层。通常，膜联蛋白-1似乎集中在细胞周围，特别是棘细胞层富含张力丝/桥粒的角质形成细胞。在高倍镜下，膜联蛋白-1出现与不同的结构，并在强烈染色的外泌汗腺导管角质形成细胞，细胞是非常高度富集的角蛋白张力丝的外观非常颗粒。在某些角质形成细胞中富含张力细丝的这种显著分布表明膜联蛋白-1在细胞骨架功能中的作用。
Annexin-1 (also called lipocortin-1 or p35), a putative substrate of the epidermal growth factor/receptor kinase, protein kinase C, and transglutaminase, was immunolocalized in embryonic, neonatal, adult, and diseased human epidermis. In embryonic skin intense annexin-1 immunoreactivity was found in the periderm at 54 d estimated gestational age (EGA). Later (EGA = 91-143 d), annexin-1 immunoreactivity was restricted to basal keratinocytes. In neonatal skin, basal cells were often more heavily stained than were suprabasal keratinocytes, which were also stained. Only basal keratinocytes stained in adult plantar skin, but in thin skin annexin-1 was present in the basal, suprabasal, and sometimes even in the granular layers of the epidermis. Often, annexin-1 appeared concentrated around the perimeter of cells, especially tonofilament/desmosome-rich keratinocytes of the spinous-cell layer. At high magnification, annexin-1 appeared associated with distinct structures and was very granular in appearance in the intensely stained ductal keratinocytes of eccrine sweat glands, cells that are very highly enriched in keratin tonofilaments. This striking distribution in certain keratinocytes enriched in tonofilaments suggests a role for annexin-1 in cytoskeletal functions.