Immunocytochemical localization of calmodulin and a heat-labile calmodulin-binding protein (CaM-BP80) in basal ganglia of mouse brain.
Immunocytochemical localization of calmodulin and a heat-labile calmodulin-binding protein (CaM-BP80) in basal ganglia of mouse brain.
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DOI：
10.1083/jcb.84.1.66
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发表时间：
1980-01
期刊：
影响因子：
--
通讯作者：
Cheung WY
中科院分区：
文献类型：
--
作者：
Wood JG;Wallace RW;Whitaker JN;Cheung WY
钙调蛋白（一种Ca2%依赖性调节蛋白）和热不稳定钙调蛋白结合蛋白的抗血清已被用于定位小鼠尾壳核中的这些蛋白。这两种蛋白质似乎位于该大脑区域的相同部位。在光镜下，钙调素和钙调素结合蛋白存在于大细胞的细胞质和突起中。在电子显微镜水平上，蛋白质仅与神经元元件相关，主要在神经元胞体和树突内的突触后位点。在树突内，免疫细胞化学标记主要与突触后密度和树突微管相关。这一结果与近年来脑和分裂细胞中钙调素的生化和免疫组织化学研究结果雅阁。因此，钙调素和热不稳定钙调素结合蛋白可能在神经系统中的神经递质作用位点和微管功能水平上发挥作用。
Antisera to calmodulin, a Ca2%-dependent modulator protein, and a heat- labile calmodulin-binding protein have been used to localize these proteins in mouse caudate-putamen. The two proteins appear to be located at identical sites in this brain area. At the light microscopic level, calmodulin and calmodulin-binding protein are found within the cytoplasm and processes of large cells. At the electron microscopic level the proteins are associated with neuronal elements only, primarily at postsynaptic sites within neuronal somata and dendrites. Within the dendrites the immunocytochemical label is associated predominantly with the postsynaptic density and dendritic microtubules. These results are in accord with recent biochemical and immunihistochemical studies of calmodulin in brain and in dividing cells. Thus, calmodulin and the heat-labile calmodulin-binding protein may play a role in the nervous system at the site of neurotransmitter action and at the level of microtubular function.