Selection for the G4 DNA Motif at the 5′ End of Human Genes
Selection for the G4 DNA Motif at the 5′ End of Human Genes
复制标题
DOI：
10.1002/mc.20496
复制
发表时间：
2009-04-01
影响因子：
4.6
通讯作者：
Maizels, Nancy
中科院分区：
文献类型：
--
作者：
Eddy, Johanna;Maizels, Nancy
G4DNA的形成可能发生在复制和转录过程中，并导致基因组的不稳定。我们定量了G4基序的丰度和人类基因转录本5‘外显子和内含子的非模板链形成G4DNA的潜力。我们发现，对于所有人类基因，G4基序相对于下游区域富含在转录产物的5‘端区域，相对于编码区在5’调控区域富含G4基序。值得注意的是，尽管肿瘤抑制基因被耗尽，原癌基因富含G4基序，但这些类别基因转录本的5‘区域G4基序的丰度没有区别。这些结果支持了G4基序在人类基因组中处于选择状态的假设。他们进一步表明，对于肿瘤抑制基因和原癌基因，独立选择决定了转录产物5‘调控区和下游编码区的G4DNA形成的可能性。(C)2009年Wiley-Liss，Inc.
Formation of G4 DNA may occur in the course of replication and transcription, and contribute to genomic instability. We have quantitated abundance of G4 motifs and potential for G4 DNA formation of the nontemplate strand of 5' exons and introns of transcripts of human genes. We find that, for all human genes, G4 motifs are enriched in 5' regions of transcripts relative to downstream regions; and in 5' regulatory regions relative to coding regions. Notably, although tumor suppressor genes are depleted and proto-oncogenes enriched in G4 motifs, abundance of G4 motifs in the 5' regions of transcripts of genes in these categories does not differ. These results support the hypothesis that G4 motifs are under selection in the human genome. They further show that for tumor suppressor genes and proto-oncogenes, independent selection determines potential for G4 DNA formation of 5' regulatory regions of transcripts and downstream coding regions. (C) 2009 Wiley-Liss, Inc.