A comparison of shock wave and sinusoidal-focused ultrasound-induced localized transfection of HeLa cells
A comparison of shock wave and sinusoidal-focused ultrasound-induced localized transfection of HeLa cells
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DOI：
10.1016/s0301-5629(99)00099-x
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发表时间：
1999-11-01
影响因子：
2.9
通讯作者：
Pfisterer, P
中科院分区：
文献类型：
--
作者：
Huber, PE;Jenne, J;Pfisterer, P
冲击波和正弦连续波超声都能介导DNA进入细胞，不同超声方式的相对转染效率尚不清楚。本文旨在比较体外碎石冲击波和聚焦正弦超声的转染效率。用β-半乳糖苷酶和荧光素酶质粒DNA报告基因转染HeLa细胞，通过电磁声源产生冲击波，在13、16或19 kV电容器电压下以1 Hz脉冲频率给予60至360个脉冲。正弦聚焦超声由以脉冲模式操作的1.18MHz的载波频率的单焦点压电陶瓷空气背衬盘换能器产生。与仅与DNA混合的细胞相比，冲击波在5%的细胞存活率下诱导高达8倍的转染细胞，而正弦聚焦超声在45%的细胞存活率下诱导高达80倍的转染细胞。HeLa细胞的相应转染效率为0.08%的冲击波和3%的聚焦超声。这些结果可能有助于选择超声方式作为一个本地化的，非侵入性的和安全的工具，介导基因转移。(C)1999年世界医学和生物学超声联合会。
Both shock waves and sinusoidal continuous wave ultrasound can-mediate DNA transfer into cells, The relative transfection efficiencies of different ultrasound modalities are unclear. The purpose of this paper is to compare the transfection efficiency of lithotripter shock waves and focused sinusoidal ultrasound in vitro. HeLa cells were transfected with beta-galactosidase and luciferase plasmid DNA reporter, Shock waves were generated by an electromagnetic sound source, Sixty to 360 pulses at 1 Hz pulse frequency were administered at 13, 16 or 19 kV capacitor voltage. Sinusoidal focused ultrasound was generated by a single focus piezoceramic air-backed disk transducer at a carrier frequency of 1.18 MHz operated in a pulsed mode, Compared to cells mixed with DNA only, shock waves induced up to eightfold more transfected cells at a cell viability of 5%, while sinusoidal-focused ultrasound induced up to 80-fold more transfected cells at a cell viability of 45%, The corresponding transfection efficiencies of the HeLa cells were 0.08% for shock waves and 3% for focused ultrasound. These results may contribute to the selection of the ultrasound modality as a localized, noninvasive and safe tool to mediate gene transfer. (C) 1999 World Federation for Ultrasound in Medicine & Biology.