Microscale Transport and Sorting by Kinesin Molecular Motors
Microscale Transport and Sorting by Kinesin Molecular Motors
复制标题
驱动蛋白分子马达的微尺度运输和分类
作者：
Lili Jia;Samira Moorjani;T. Jackson;W. Hancock
随着生物分子检测系统尺寸的缩小，对在微米和纳米尺度上传输和定位材料的系统的需求不断增加。我们的目标是将细胞运输机械（驱动蛋白分子马达和微管）与集成光电器件结合到混合生物/工程微型设备中，该设备将结合、运输和检测特定蛋白质、DNA/RNA 分子、病毒或细胞。对于微尺度运输，使用 SU-8 光刻胶在玻璃上创建 1.5 μm 深的通道，驱动蛋白马达吸附在通道底部，通道壁用于弯曲和重定向在固定马达上移动的微管。研究了新颖的通道几何形状，作为对在这些通道中移动的微管进行重定向和分类的手段。我们证明直流和交流电场足以在溶液中运输微管，建立了一种重新引导微管在通道中移动的方法。最后，我们反转了几何结构，证明驱动蛋白可以沿着表面固定的微管运输金纳米线，为纳米级定向组装提供了模型。
As biomolecular detection systems shrink in size, there is an increasing demand for systems that transport and position materials at micron- and nanoscale dimensions. Our goal is to combine cellular transport machinery—kinesin molecular motors and microtubules—with integrated optoelectronics into a hybrid biological/engineered microdevice that will bind, transport, and detect specific proteins, DNA/RNA molecules, viruses, or cells. For microscale transport, 1.5 μm deep channels were created with SU-8 photoresist on glass, kinesin motors adsorbed to the bottom of the channels, and the channel walls used to bend and redirect microtubules moving over the immobilized motors. Novel channel geometries were investigated as a means to redirect and sort microtubules moving in these channels. We show that DC and AC electric fields are sufficient to transport microtubules in solution, establishing an approach for redirecting microtubules moving in channels. Finally, we inverted the geometry to demonstrate that kinesins can transport gold nanowires along surface immobilized microtubules, providing a model for nanoscale directed assembly.