Dynamics of noise-induced cellular injury and repair in the mouse cochlea
Dynamics of noise-induced cellular injury and repair in the mouse cochlea
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DOI：
10.1007/s101620020028
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发表时间：
2002-09-01
期刊：
影响因子：
--
通讯作者：
Liberman, MC
中科院分区：
文献类型：
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作者：
Wang, Y;Hirose, K;Liberman, MC
为了评估噪声诱导的组织损伤和修复的动力学，CBA/CAJ小鼠分组暴露在94、100、106、112或116dBSPL水平的八度频带噪声中2小时，并在0、12、24小时或1、2或8周的存活时间进行评估。通过听性脑干反应(ABR)评估功能变化，范围从可逆性阈值漂移(94dB)到严重的永久性丧失(116dB)。对耳蜗管内的大多数主要细胞类型，包括毛细胞(及其立体纤毛)、支持细胞、神经节细胞、螺旋韧带纤维细胞、螺旋缘纤维细胞和血管纹，进行了光镜下组织病理学检查。形态计量学允许系统地评估损伤模式，作为(1)耳蜗位置、(2)暴露水平和(3)暴露后存活的函数。对急性和慢性噪声引起的细胞损伤的机制进行了深入的讨论。
To assess the dynamics of noise-induced tissue injury and repair, groups of CBA/CaJ mice were exposed to an octave-band noise for 2 hours at levels of 94, 100, 106, 112, or 116 dB SPL and evaluated at survival times of 0, 12, 24 hours or 1, 2, or 8 weeks. Functional change, assessed via auditory brainstem response (ABR), ranged from a reversible threshold shift (at 94 dB) to a profound permanent loss (at 116 dB). Light microscopic histopathology was assessed in serial thick plastic sections and involved quantitative evaluation of most major cell types within the cochlear duct, including hair cells (and their stereocilia), supporting cells, ganglion cells, spiral ligament fibrocytes, spiral limbus fibrocytes, and the stria vascularis. Morphometry allowed patterns of damage to be systematically assessed as functions of (1) cochlear location, (2) exposure level, and (3) postexposure survival. Insights into mechanisms of acute and chronic noise-induced cellular damage are discussed.