Detailed characterisation of the flow resistance of commercial sub-2 μm reversed-phase columns
Detailed characterisation of the flow resistance of commercial sub-2 μm reversed-phase columns
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DOI：
10.1016/j.chroma.2007.11.086
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发表时间：
2008-01-12
影响因子：
4.1
通讯作者：
Desmet, Gert
中科院分区：
文献类型：
--
作者：
Cabooter, Deirdre;Billen, Jeroen;Desmet, Gert
使用最近推出的总孔阻塞方法测定外部孔隙率，并使用扫描电子显微镜图片测量颗粒的实际尺寸，研究了三个不同制造商但具有相同表面化学性质（C-18）的六个亚2μm柱的压降特性。使用索特平均粒径来正确解释粒径分布，并校正颗粒内孔隙率的影响后，发现所有色谱柱产生的 Kozeny-Carman 常数 (f(KC)) 值接近 180，与理论一致。随后，该协议可用于量化不同的系统参数，例如平均粒径、填充密度和颗粒内孔隙率（所有参数在制造商之间都有很大差异）对观察到的总床渗透率的影响。与 f(KC)=180 值的小（向上）偏差可能与较大的粒度分布宽度相关，更值得注意的是，与颗粒批次中存在的最大与最小颗粒的高尺寸比相关。 (c) 2007 Elsevier B.V. 保留所有权利。
The pressure-drop characteristics of six sub-2 mu m columns of three different manufacturers but with the same surface chemistry (C-18) have been studied using the recently introduced total pore blocking method for the determination of the external porosity and by using scanning electron microscopy pictures to measure the actual size of the particles. Having used the Sauter-mean particle size to correctly account for the particle size spread, and having corrected for the influence of the intra-particle porosity, it was found that all columns yielded Kozeny-Carman constant (f(KC)) values close to 180, in agreement with the theory. This agreement could subsequently be used to quantify how the different system parameters such as mean particle size, packing density and intra-particle porosity (all tending to vary significantly from manufacturer to manufacturer) each contributes to the observed total bed permeability. Small (upward) deviations from the f(KC)=180-value could be correlated to a larger width of the particle size distribution, and more notably to the existence of a high size ratio of the largest to the smallest particles present in the particle batches. (c) 2007 Elsevier B.V. All rights reserved.