Gene expression in skeletal tissues: application of laser capture microdissection
Gene expression in skeletal tissues: application of laser capture microdissection
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DOI：
10.1111/j.1365-2818.2005.01511.x
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发表时间：
2005-10-01
影响因子：
2
通讯作者：
Shur, I
中科院分区：
文献类型：
--
作者：
Benoyahu, D;Akavia, UD;Shur, I
组织分化基于调节特定表型的转录因子、细胞因子受体和核受体的表达。本研究的目的是从各种骨骼组织中选择细胞，以分析细胞在体内天然环境中的差异基因表达。从骨骼组织如软骨、骨膜、骨和肌肉中获得细胞是一项困难的任务，这些组织被结构化在一起并且不作为单独的器官存在。我们使用激光捕获显微切割，它允许从组织切片中选择和分离单个细胞。从这些组织中分离的RNA用于逆转录酶-聚合酶链反应进行分子分析。我们分析了转录因子（cFOS，cbfa 1，MyoD），细胞因子受体，核受体，碱性磷酸酶和结构蛋白骨钙素和胶原蛋白II的表达。所获得的结果表明，根据在其原生的体内环境中的组织排列的基因表达的差异模式，与完整的骨骼组织生理学的背景下的分析基因的功能的可靠解释。
Tissue differentiation is based on the expression of transcription factors, receptors for cytokines, and nuclear receptors that regulate a specific phenotype. The purpose of this study was to select cells from various skeletal tissues in order to analyse differential gene expression of cells in the native environment in vivo. It is a difficult task to obtain cells from skeletal tissues, such as cartilage, periost, bone and muscle, that are structured together and do not exist as individual organs. We used laser capture microdissection which permits the selection and isolation of individual cells from tissue sections. The RNA isolated from these tissues was used for reverse transcriptase-polymerase chain reactions for molecular analysis. We analysed the expression of transcription factors (cFOS, cbfa1, MyoD), receptors for cytokines, nuclear receptors, alkaline phosphatase and the structural proteins osteocalcin and collagen II. The results obtained demonstrate differential patterns of gene expression according to the tissue arrangement in their native in vivo environment, with reliable interpretation of the functions of the analysed genes in the context of intact skeletal tissue physiology.