Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress.
Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress.
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DOI：
10.1111/mec.13609
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发表时间：
2016-05
影响因子：
4.9
通讯作者：
Besansky NJ
中科院分区：
文献类型：
--
作者：
Uyhelji HA;Cheng C;Besansky NJ
渗透调节系统的进化是物种在淡水和咸水生境之间过渡的关键因素。Coluzzii按蚊和An.Merus是狭盐性和广盐性疟疾媒介蚊子，属于更大的兄弟物种冈比亚按蚊复合体，在过去200万年内在非洲辐射。这些媒介物种的比较生态基因组学可以洞察允许这种物种复合体快速辐射到盐度反差的栖息地的机制。在这里，我们使用RNA-Seq来研究An.Coluzzii和An.Merus在短暂地将每个物种的幼龄和老年幼虫暴露在咸水(SW)或淡水(FW)中之后。我们的检查目的是确定候选基因和途径，负责更大的西南耐受性的AN。梅鲁斯。我们的结果与基因诱导调节耐盐性的能力是一致的，随着盐浓度的增加，两个物种都表现出越来越多的差异基因表达。除了离子转运体如AgAE2可能作为渗透调节的效应物外，我们还发现丝裂原激活的蛋白激酶可能在响应盐度的磷酸化信号通路中发挥作用，并报道了蚊子免疫反应和渗透调节之间的潜在串扰。这项研究为应用这些疟疾媒介日益增长的分子知识以提高对它们的生态耐受性和栖息地占有率的了解迈出了关键的一步。
Evolution of osmoregulatory systems is a key factor in the transition of species between fresh- and saltwater habitats. Anopheles coluzzii and An. merus are stenohaline and euryhaline malaria vector mosquitoes belonging to a larger group of sibling species, the Anopheles gambiae complex, which radiated in Africa within the last 2 million years. Comparative ecological genomics of these vector species can provide insight into the mechanisms that permitted the rapid radiation of this species complex into habitats of contrasting salinity. Here we use RNA-Seq to investigate gene expression differences between An. coluzzii and An. merus after briefly exposing both young and old larval instars of each species to either saltwater (SW) or freshwater (FW). Our examination aims to identify candidate genes and pathways responsible for the greater SW tolerance of An. merus. Our results are congruent with the ability of gene induction to mediate salinity tolerance, with both species showing increasing amounts of differential gene expression between SW and FW as salt concentrations increase. Besides ion transporters such as AgAE2 that may serve as effectors for osmoregulation, we also find mitogen activated protein kinases that may serve in a phosphorylation signaling pathway responding to salinity, and report potential cross-talk between the mosquito immune response and osmoregulation. This study provides a key step towards applying the growing molecular knowledge of these malaria vectors to improve understanding of their ecological tolerances and habitat occupancy.