MMP-2 assay within the hybrid layer created by a two-step etch-and-rinse adhesive: Biochemical and immunohistochemical analysis
MMP-2 assay within the hybrid layer created by a two-step etch-and-rinse adhesive: Biochemical and immunohistochemical analysis
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DOI：
10.1016/j.jdent.2011.04.004
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发表时间：
2011-07-01
影响因子：
4.4
通讯作者：
Breschi, Lorenzo
中科院分区：
文献类型：
--
作者：
Mazzoni, Annalisa;Carrilho, Marcela;Breschi, Lorenzo
目的：混合层（HL）的降解树脂渗透牙本质内的结果是由多种降解因素，包括特定的基质金属蛋白酶（MMP）的胶原溶解活性。因此，抑制宿主来源的MMPs可能会减缓HL的降解。零假设检验的是，MMP-2的存在是相似的，无论氯己定（CHX）预处理或使用粘合剂。方法：从离体人牙制备的牙本质粉末分为4组：（G1）矿化粉末（G2）1%磷酸处理1 min的牙本质粉;（G3）用Adper Scotchbond 1 XT（SB 1XT; 3 M ESPE）处理的1%磷酸蚀刻牙本质;（G4）用0.2% CHX处理的1%磷酸蚀刻牙本质，然后用SB 1XT处理。使用比色测定系统（QuantiSir）测定可检测的pro-MMP-2和MMP-2的浓度。结果：1%磷酸处理的牙本质粉（G2）中MMP-2的表达较对照组（G1）明显降低; SB 1XT的应用（G3）导致MMP-2的增加，而SB 1XT应用之前的0.2%CHX（G4）降低MMP-2。FEI-SEM/TEM分析显示，MMP-2分布在HL的老化接口显示增加MMP-2模式在对照组和小标签中的CHX预处理的specimens.Conclusion：本研究的结果支持使用无毒的MMPs抑制剂，如CHX，作为一个适当的附加步骤，在粘接程序中，以增加寿命的粘合剂dispensations。(C)2011爱思唯尔有限公司保留所有权利。
Objective: Degradation of hybrid layers (HLs) within resin-infiltrated dentine results from multiple degradation factors, including collagenolytic activity of specific matrix metalloproteinases (MMPs). Inhibition of host-derived MMPs may, therefore, slow the degradation of HL. The null hypothesis tested is that the presence of MMP-2 is similar regardless of chlorhexidine (CHX) pre-treatment or the use of an adhesive.Methods: Powdered dentine prepared from extracted human teeth was divided into 4 groups: (G1) mineralised powder (control group); (G2) dentine powder treated with 1% phosphoric acid for 1 min; (G3) 1% phosphoric acid-etched dentine treated with Adper Scotchbond 1 XT (SB1XT; 3M ESPE); (G4) 1% phosphoric acid-etched dentine treated with 0.2% CHX followed by SB1XT. The concentration of detectable pro-MMP-2 and MMP-2 was assayed using a colorimetric assay system (QuantiSir). In addition, the presence of MMP-2 in the HL was assessed in 1 year-aged adhesive-dentine interfaces using an immunohistochemical approach under FEI-SEM/TEM.Results: In dentine powder treated with 1% phosphoric acid (G2), MMP-2 level decreased compared to controls (G1); the application of SB1XT (G3) resulted in an increase of MMP-2, whilst 0.2% CHX before SB1XT application (G4), reduced MMP-2. The FEI-SEM/TEM analysis revealed MMP-2 distribution within the HL of aged interfaces showing increase MMP-2 patterns in the control group and minor labelling in the CHX-pretreated specimens.Conclusion: The results of this study support the use of non-toxic MMPs inhibitors, such as CHX, as an appropriate additional step in bonding procedures in order to increase the longevity of the adhesive restorations. (C) 2011 Elsevier Ltd. All rights reserved.