PURIFICATION AND CHARACTERIZATION OF A MAJOR ALLERGEN OF ALTERNARIA-ALTERNATA
PURIFICATION AND CHARACTERIZATION OF A MAJOR ALLERGEN OF ALTERNARIA-ALTERNATA
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DOI：
10.1016/0161-5890(91)90154-c
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发表时间：
1991-04-01
影响因子：
3.6
通讯作者：
MONTAGUE, AE
中科院分区：
文献类型：
--
作者：
DEARDS, MJ;MONTAGUE, AE
用SDS-PAGE和免疫印迹方法鉴定了链格孢霉的主要变应原Alt a Bd29K。采用凝胶渗透和离子交换高效液相色谱分离纯化。表观分子质量为29,000，半胱氨酸连接的亚基为15,000 M(R)。表观等电点为4.2。它被发现是Alt I过敏原组的一种成分。
A major allergen of Alternaria (Alt a Bd29K) was identified by SDS-PAGE and immunoblotting. It was purified by gel permeation and ion-exchange HPLC. The apparent molecular mass was 29,000, with a cysteine linked sub-unit of 15,000 M(r). The apparent isoelectric point was 4.2. It was found to be a constituent of the Alt I allergen group.