Transcriptomic and Expression Analysis of the Salivary Glands in White-Backed Planthoppers, Sogatella furcifera.
Transcriptomic and Expression Analysis of the Salivary Glands in White-Backed Planthoppers, Sogatella furcifera.
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白背飞虱唾液腺转录组及表达分析
DOI：
10.1371/journal.pone.0159393
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Hou ML
中科院分区：
文献类型：
--
作者：
Li Z;An XK;Liu YD;Hou ML
白背飞虱（Sogatella furcifera, Horváth）是危害水稻健康的主要害虫之一。白头鲂的唾液腺在摄食行为中起着重要作用。然而，目前对唾液腺的分子水平了解甚少。我们使用Illumina测序对成年白斑小灵通的唾液腺转录组（sialotranscripome）进行了测序。从唾液腺共获得65,595个转录本和51,842个单基因。根据对Nr数据库的注释，鉴定出的许多独特基因与半翼龙唾液中研究最多的酶有关。在本研究中，我们从WBPH唾液转录组中鉴定出32个唾液蛋白基因，这些基因被归类为参与糖代谢、解毒、抑制植物防御反应、免疫相关反应、一般消化和其他植物吞噬过程。组织表达谱分析显示，32个唾液蛋白基因（多铜氧化酶4、多铜氧化酶6、羧化酯酶和尿苷磷酸化酶1异构体X2）中有4个主要在唾液腺中表达，表明它们可能在虫稻相互作用中起作用。32个唾液蛋白基因中有13个主要在肠道表达，可能在消化和解毒机制中发挥作用。发育表达谱分析显示，32个唾液蛋白基因中有26个基因的表达水平无显著差异，提示它们可能在WBPH唾液腺发育的各个阶段发挥作用。其余6个基因在成虫唾液腺中表达量较高。32个基因中有31个（除推定的乙酰胆碱酯酶1外）在雌雄成虫中无显著差异，表明其表达量在两性间无差异。本报告分析了WBPH的唾液转录组，转录组提供了参与摄食的基因的基础列表。我们的研究结果将有助于研究WBPH与寄主植物之间的相互作用机制。
The white-backed planthopper (WBPH), Sogatella furcifera (Horváth), is one of the serious rice pests because of its destructive feeding. The salivary glands of the WBPH play an important role in the feeding behaviour. Currently, however, very little is known about the salivary glands at the molecular level. We sequenced the salivary gland transcriptome (sialotranscripome) of adult WBPHs using the Illumina sequencing. A total of 65,595 transcripts and 51,842 unigenes were obtained from salivary glands. According to annotations against the Nr database, many of the unigenes identified were associated with the most studied enzymes in hemipteran saliva. In the present study, we identified 32 salivary protein genes from the WBPH sialotranscripome, which were categorized as those involved in sugar metabolism, detoxification, suppression of plant defense responses, immunity-related responses, general digestion, and other phytophagy processes. Tissue expression profiles analysis revealed that four of 32 salivary protein genes (multicopper oxidase 4, multicopper oxidase 6, carboxylesterase and uridine phosphorylase 1 isform X2) were primarily expressed in the salivary gland, suggesting that they played putative role in insect-rice interactions. 13 of 32 salivary protein genes were primarily expressed in gut, which might play putative role in digestive and detoxify mechanism. Development expression profiles analysis revealed that the expression level of 26 of 32 salivary protein genes had no significant difference, suggesting that they may play roles in every developmental stages of salivary gland of WBPH. The other six genes have a high expression level in the salivary gland of adult. 31 of 32 genes (except putative acetylcholinesterase 1) have no significant difference in male and female adult, suggesting that their expression level have no difference between sexes. This report analysis of the sialotranscripome for the WBPH, and the transcriptome provides a foundational list of the genes involved in feeding. Our data will be useful to investigate the mechanisms of interaction between the WBPH and the host plant.