Isolation of follicular dendritic cells from human tonsils and adenoids. II. Immunocytochemical characterization
Isolation of follicular dendritic cells from human tonsils and adenoids. II. Immunocytochemical characterization
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从人扁桃体和腺样体中分离滤泡树突状细胞。
DOI：
10.1002/eji.1830140312
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发表时间：
1984
影响因子：
5.4
通讯作者：
L. Simar
中科院分区：
文献类型：
--
作者：
E. Heinen;C. Lilet;D. Mason;H. Stein;J. Boniver;D. Radoux;C. Kinet‐Denoël;L. Simar
滤泡树突状细胞（FDC）是仅在淋巴滤泡内发现的特化细胞。它们结合免疫复合物，在向滤泡B细胞呈递抗原和生成B细胞记忆中发挥作用。本报告描述了从人扁桃体和腺样体中分离FDC的情况。这些分离的细胞具有不寻常的球形排列，并将淋巴细胞包裹在其膜的延伸内。它们的超微结构特征与原位观察到的相似。通过免疫荧光和胶体金免疫染色（在电镜水平上）分析了分离的FDC与许多单克隆抗体的反应性。与之前的组织切片研究结果一致，分离的FDC表面可以检测到IgM、IgG和IgA（但不包括IgD）， C3b受体和FDC相关抗原也可以通过单克隆抗体R4/23检测到。与FDC相关的免疫球蛋白大多包埋在电子致密材料中。包裹在FDC膜延伸内的淋巴样细胞多数为B细胞型。这些结果表明，分离的FDC可能适合进一步的体外研究其在体液免疫应答中的作用。
Follicular dendritic cells (FDC) are specialized cells found only within lymphoid follicles. They bind immune complexes and play a role in the presentation of antigen to follicular B cells and in the generation of B cell memory. In the present report the isolation of FDC from human tonsils and adenoids is described. These isolated cells have an unusual spherical arrangement and enclose lymphocytes within extensions of their membranes. Their ultrastructural features are similar to those observed in situ. The reactivity of isolated FDC with a number of monoclonal antibodies was analyzed by immunofluorescence and by immunostaining (at the electron microscopic level) with colloidal gold. In keeping with the results of previous investigations on tissue sections IgM, IgG and IgA (but not IgD) can be detected on the surface of isolated FDC, as can C3b receptors and the FDC‐associated antigen detected by monoclonal antibody R4/23. The immunoglobulins associated with FDC are mostly embedded in an electron‐dense material. The majority of the lymphoid cells enclosed within the membrane extensions of FDC are of B cell type. These results suggest that isolated FDC may be suitable for further in vitro investigation of their role in the humoral immune response.
B 淋巴细胞在谱系发育和免疫反应中的分化。
DOI：
--
发表时间：
1981
期刊：
Progress in allergy
影响因子：
--
作者：
Hammerling,U
通讯作者：
Hammerling,U