High-affinity binding of granulocyte-macrophage colony-stimulating factor to normal and leukemic human myeloid cells.
High-affinity binding of granulocyte-macrophage colony-stimulating factor to normal and leukemic human myeloid cells.
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粒细胞-巨噬细胞集落刺激因子与正常和白血病人骨髓细胞的高亲和力结合。
DOI：
10.1073/pnas.83.3.669
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发表时间：
1986
影响因子：
11.1
通讯作者：
Golde,DW
中科院分区：
文献类型：
--
作者：
Gasson,JC;Kaufman,SE;Weisbart,RH;Tomonaga,M;Golde,DW
纯化的天然和生物合成（重组）人粒细胞-巨噬细胞集落刺激因子（GM-CSF）刺激骨髓祖细胞集落形成，增强成熟中性粒细胞的功能。这两种作用都发生在浓度1 - 100pm之间，在10- 20pm有一半的最大刺激。我们已经研究了125i标记的GM-CSF在这个浓度范围内与反应性靶细胞的特异性结合。结果显示gm - csf反应性白血病细胞（KG-1, HL-60）和正常供者外周血中性粒细胞上有少量（50-250）高亲和力（15- 30pm）结合位点。这种高亲和力的结合成分在无反应的细胞系中不存在（KG-1a, K562）。这些结果表明，该结合位点介导了GM-CSF对骨髓细胞增殖和功能的生物学活性。
Purified natural and biosynthetic (recombinant) human granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulate colony formation by myeloid progenitor cells and enhance the function of mature neutrophils. Both of these actions occur at concentrations between 1 and 100 pM, with half-maximal stimulation at 10-20 pM. We have examined specific binding of 125I-labeled GM-CSF to responsive target cells in this range of concentrations. The results show a low number (50-250) of high-affinity (15-30 pM) binding sites on GM-CSF-responsive leukemic cells (KG-1, HL-60), as well as on peripheral blood neutrophils from normal donors. This high-affinity binding component was absent from unresponsive cell lines (KG-1a, K562). These results suggest that this binding site mediates the biological activities of GM-CSF on both proliferation and function of myeloid cells.