Culture of mouse prostatic epithelial cells from genetically engineered mice
Culture of mouse prostatic epithelial cells from genetically engineered mice
复制标题
DOI：
10.1002/pros.20193
复制
发表时间：
2005-05-15
期刊：
影响因子：
2.8
通讯作者：
Cramer, SD
中科院分区：
文献类型：
--
作者：
Barclay, WW;Cramer, SD
背景。缺乏合适的前列腺癌模型是前列腺癌研究的一个主要问题。在开发更好的前列腺疾病啮齿动物体内遗传模型方面取得了进展。然而，体外模型通常更适合于阐明与该疾病有关的细胞机制。我们对年轻雄性小鼠的四个前列腺叶进行显微解剖，收集上皮成分，并从这些组织中培养上皮细胞。我们在长期和三维培养中保持了这些细胞的生长。我们从多种小鼠遗传品系中可重复性地收获和培养延长传代小鼠前列腺上皮细胞（mpec）。这些细胞表达管腔和基底上皮标记物以及雄激素受体。此外，mpec在三维胶原基质中形成经典的分支结构。我们开发了一种新的培养系统，可以在长期培养中收获和培养mpec。这些细胞将作为前列腺疾病小鼠遗传模型研究的有益体外补充。(c) 2004 Wiley-Liss。公司。
BACKGROUND. The lack of appropriate prostate cancer models is a major problem for prostate cancer research. Progress has been made towards the development of better in vivo rodent genetic models for prostatic disease. However, an in vitro model is often preferred for the elucidation of cellular mechanisms involved in the disease.METHODS. We microdissected the four prostatic lobes from young male mice, harvested the epithelial components, and grew epithelial cells from these tissues. We maintained the growth of these cells in long-term and three-dimensional culture.RESULTS. We have reproducibly harvested and cultured for extended passages mouse prostatic epithelial cells (MPECs) from a variety of mouse genetic strains. These cells express luminal and basal epithelial markers as well as the androgen receptor. Additionally, MPECs form classic branching structures in a three-dimensional collagen matrix.CONCLUSIONS. We have developed a novel culture system to harvest and grow MPECs in long-term culture. These cells will serve as a useful in vitro complement to studies using mouse genetic models for prostatic disease. (c) 2004 Wiley-Liss. Inc.