Mechanotransduction in bone: Osteoblasts are more responsive to fluid forces than mechanical strain
Mechanotransduction in bone: Osteoblasts are more responsive to fluid forces than mechanical strain
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DOI：
10.1152/ajpcell.1997.273.3.c810
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发表时间：
1997-09-01
影响因子：
5.5
通讯作者：
Duncan, RL
中科院分区：
文献类型：
--
作者：
Owan, I;Burr, DB;Duncan, RL
施加于骨的机械力在细胞上产生两个局部机械信号：细胞外基质的变形（基质应变）和细胞外液流动。为了研究这些刺激对成骨细胞的影响，MC 3 T3-E1细胞在I型胶原包被的塑料板上生长，并进行四点弯曲。该技术在细胞上产生均匀水平的生理应变和流体力。这些参数中的每一个都可以独立地变化。骨桥蛋白（OPN）mRNA表达用于评估MC 3 T3-E1细胞的合成代谢反应。当流体力较低时，应变幅度和应变率均与OPN表达无关。然而，更高量级的流体力显著增加了OPN信息水平，与应变量级或速率无关。这些数据表明，流体力，而不是机械拉伸，影响成骨细胞中的OPN表达，并表明骨基质内细胞外液流动诱导的流体力可能在响应机械负荷的骨形成中起重要作用。
Mechanical force applied to bone produces two localized mechanical signals on the cell: deformation of the extracellular matrix (substrate strain) and extracellular fluid flow. To study the effects of these stimuli on osteoblasts, MC3T3-E1 cells were grown on type I collagen-coated plastic plates and subjected to four-point bending. This technique produces uniform levels of physiological strain and fluid forces on the cells. Each of these parameters can be varied independently. Osteopontin (OPN) mRNA expression was used to assess the anabolic response of MC3T3-E1 cells. When fluid forces were low, neither strain magnitude nor strain rate was correlated with OPN expression. However, higher-magnitude fluid forces significantly increased OPN message levels independently of the strain magnitude or rate. These data indicate that fluid forces, and not mechanical stretch, influence OPN expression in osteoblasts and suggest that fluid forces induced by extracellular fluid flow within the bone matrix may play an important role in bone formation in response to mechanical loading.