Mushroom body defect, a gene involved in the control of neuroblast proliferation in Drosophila, encodes a coiled-coil protein
Mushroom body defect, a gene involved in the control of neuroblast proliferation in Drosophila, encodes a coiled-coil protein
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DOI：
10.1073/pnas.97.14.8122
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发表时间：
2000-07-05
影响因子：
11.1
通讯作者：
Raabe, T
中科院分区：
文献类型：
--
作者：
Guan, ZH;Prado, A;Raabe, T
神经发生依赖于神经母细胞（神经元前体细胞）的适当数量和精确控制的增殖的建立。蘑菇体缺陷（mud）基因在成神经细胞行为的这两个方面的作用，以及在果蝇生物学的其他方面可能的作用，是由与mud突变相关的表型所暗示的。我们通过确定一个点突变体中的序列变化来定位泥浆，确定受突变影响的预测ORF，并显示基因组的适当片段拯救泥浆突变体表型。泥cDNA的分析和泥成绩单的调查北方印迹表明，该基因是受差异剪接，主要是在胚胎发生过程中表达，但也在较低的水平，在随后的发展阶段中的性二态性的方式。该基因被预测编码一种多肽，没有明显的同源物，但具有两个突出的结构特征，一个长的卷曲螺旋，构成蛋白质的中央核心和羧基末端跨膜结构域。
Neurogenesis relies on the establishment of the proper number and precisely controlled proliferation of neuroblasts, the neuronal precursor cells. A role for the mushroom body defect (mud) gene in both of these aspects of neuroblast behavior, as well as possible roles in other aspects of fruit fly biology, is implied by phenotypes associated with mud mutations. We have localized mud by determining the sequence change in one point mutant, identifying a predicted ORF affected by the mutation, and showing that an appropriate segment of the genome rescues mud mutant phenotypes. An analysis of mud cDNAs and a survey of mud transcripts by Northern blotting indicate that the gene is subject to differential splicing and is expressed primarily during embryogenesis but also, at lower levels, during subsequent developmental stages in a sexually dimorphic manner. The gene is predicted to encode a polypeptide without obvious homologs but with two prominent structural features, a long coiled coil that constitutes the central core of the protein and a carboxyl-terminal transmembrane domain.