Heightened Interferon-γ Production by Mononuclear Cells from Bladder Cancer Patients
Heightened Interferon-γ Production by Mononuclear Cells from Bladder Cancer Patients
复制标题
膀胱癌患者的单核细胞产生更多的干扰素-γ
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
W. Catalona
中科院分区：
文献类型：
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作者：
A. Shapiro;T. Ratliff;D. R. Kelley;W. Catalona
Abstract Interferon-γ (IFN-γ) production by peripheral blood leukocytes from bladder cancer patients was compared with that of patients with prostate cancer and benign prostatic hyperplasia, nontumor-bearing patients with bacterial infections, and normal controls. Leukocyte preparations including mononuclear cells isolated on a Ficoll-Hypaque density gradient (Fraction 2) and glassnonadherent mononuclear cells (Fraction 3) were stimulated with Protein A from Staphylococcus aureus, and IFN-γ production was monitored 24 hr later. The class of interferon produced was identified by antibody neutralization experiments which clearly showed S. aureus Protein A-induced interferon to be IFN-γ. There was significantly heightened IFN-γ production by Fraction 3 cells from bladder cancer patients and patients with bacterial infections. Heightened IFN-γ production by bladder cancer patients was not observed in the Fraction 2 cells. No correlation was observed between IFN-γ production and patients with invasive or noninvasive bladder cancer, but IFN-γ production was lower in patients having Stage C or D tumors than in those having Stage A or B tumors. These results in conjunction with previous reports demonstrating heightened IFN-γ production during periods of antigenic stimulation suggest that bladder tumors may induce a cell-mediated immune response in the host as evidenced by the elevation in IFN-γ production. Moreover, the results suggest that macrophages may be important regulators of IFN-γ production in bladder cancer patients.
