Glucose-6-phosphate dehydrogenase is required for extracellular polysaccharide production, cell motility and the full virulence of Xanthomonas oryzae pv. oryzicola
Glucose-6-phosphate dehydrogenase is required for extracellular polysaccharide production, cell motility and the full virulence of Xanthomonas oryzae pv. oryzicola
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6-磷酸葡萄糖脱氢酶是米黄单胞菌的细胞外多糖产生、细胞运动和充分毒力所必需的。
DOI：
10.1016/j.micpath.2014.11.007
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发表时间：
2015-01-01
影响因子：
3.8
通讯作者：
Chen, Gong-You
中科院分区：
文献类型：
--
作者：
Guo, Wei;Zou, Li-Fang;Chen, Gong-You
葡萄糖-6-磷酸脱氢酶（Zwf）在生物体中催化6-磷酸葡萄糖转化为6-磷酸葡萄糖酸酯，用于enterner - doudoroff （ED）和戊糖磷酸途径。然而，zwf编码基因是否参与植物致病菌的发病机制尚不清楚。在这篇报道中，我们发现了米黄单胞菌pv的zwf缺失突变。oryzicola （Xoc）导致病原菌不能有效利用葡萄糖、蔗糖、果糖、甘露糖和半乳糖进行生长。葡萄糖、蔗糖、果糖、甘露糖和半乳糖对zwf转录本水平的诱导作用强于NY培养基（无糖）。zwf的缺失突变还改变了扩散信号因子（DSF）信号网络中rpfF、rpfG和clp等关键基因的转录水平。此外，zwf的缺失突变使水稻叶片中细菌的毒力和生长能力受损，细菌的细胞活力和胞外多糖（EPS）的产生减少。由于在trans中存在zwf， zwf缺失突变体中上述丧失的性状完全恢复到野生型水平。所有这些结果表明，zwf通过参与影响细菌dsf信号网络的碳水化合物代谢，在水稻叶片中发挥Xoc的全部毒力。(C) 2014 Elsevier Ltd.版权所有。
Glucose-6-phosphate dehydrogenase (Zwf) catalyzes conversion of glucose 6-phosphate into gluconate 6-phosphate for Entner-Doudoroff (ED) and pentose phosphate pathways in living organisms. However, it is unclear whether the Zwf-coding gene is involved in pathogenesis of phytopathogenic bacterium. In this report, we found that deletion mutation in zwf of Xanthomonas oryzae pv. oryzicola (Xoc), led the pathogen unable to effectively utilize glucose, sucrose, fructose, mannose and galactose for growth. The transcript level of zwf was strongly induced by glucose, sucrose, fructose, mannose and galactose than that by the NY medium (non sugar). The deletion mutagenesis in zwf also altered the transcript level of key genes, such as rpfF, rpfG and clp, in diffusible signal factor (DSF)-signaling network. In addition, the deletion mutation in zwf impaired bacterial virulence and growth capability in rice leaves, reduced bacterial cell motility and extracellular polysaccharide (EPS) production. The lost properties mentioned above in the zwf deletion mutant were completely restored to the wild-type level by the presence of zwf in trans. All these results suggest that zwf is required for the full virulence of Xoc in rice leaves by involving carbohydrate metabolisms that impact bacterial DSF-signaling network. (C) 2014 Elsevier Ltd. All rights reserved.