Two Separate Genes Encode the Catalytic 70 kDa V-ATPase Subunit in Psilotum and Equisetum
Two Separate Genes Encode the Catalytic 70 kDa V-ATPase Subunit in Psilotum and Equisetum
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松叶草和木贼属中两个独立的基因编码催化 70 kDa V-ATP 酶亚基
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发表时间：
1991
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影响因子：
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通讯作者：
J. Gogarten
中科院分区：
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作者：
T. Starke;T. P. Linkkila;J. Gogarten
摘要 液泡型 ATP 酶已在多种真核细胞的内膜上发现，例如细胞内膜。溶酶体、嗜铬颗粒、源自高尔基体的囊泡、内体和液泡。尽管这种 ATP 酶类型针对一个细胞中的不同区室，但每个基因组仅发现每个亚基的一个基因。使用跨内含子-外显子边界的 PCR，我们发现两个不同的基因编码 Psilotum nudum 和 Equisetum arvense 中 V-ATPase 的催化亚基。三个密码子位置的替换率和插入序列表明，在松属植物中，这两个基因都被转录并处于选择压力下，然而，木贼属的情况似乎并非如此。与种间相似性相比，每个物种中发现的两个基因之间相对较高的相似性表明，在基因复制发生后的一段时间内，这两个拷贝受到基因转换机制的影响。注意到并统计验证了干预序列本身的意外保守程度，然而，没有检测到可以解释这些发现的结构限制。
Abstract Vacuolar type ATPases have been found on various endomem branes of eukaryotic cells, e.g. lysosomes, chromaffin granules, vesicles derived from the Golgi apparatus, endosomes and vacuoles. Although this ATPase type is targeted to different compartments in one cell, only one gene for each subunit had been found per genome. Using PCR across intron-exon boundaries we show that two different genes encode the catalytic subunit of the V-ATPase in Psilotum nudum and Equisetum arvense. The substitution rates for the three codon positions and the intervening sequences show that in Psilotum both genes are transcribed and are under selection pressure, however, this seems not to be the case for Equisetum. The relatively high similarity between the two genes found in each species as compared to the interspecies similarities suggest that for some time after the gene duplication had occurred the two copies were subject to gene conversion mechanisms. An unexpected degree of conservation of the intervening sequences themselves is noted and statistically verified, however, no structural constraints that could explain these findings were detected.