Crystallization and initial X-ray diffraction analysis of human pyruvate dehydrogenase.
Crystallization and initial X-ray diffraction analysis of human pyruvate dehydrogenase.
复制标题
人丙酮酸脱氢酶的结晶和初始 X 射线衍射分析。
DOI：
10.1107/s0907444901000427
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Patel,MS
中科院分区：
文献类型：
--
作者：
Ciszak,E;Korotchkina,LG;Hong,YS;Joachimiak,A;Patel,MS
Human pyruvate dehydrogenase (E1) is a component enzyme of the pyruvate dehydrogenase complex. The enzyme catalyzes the irreversible decarboxylation of pyruvic acid and the rate-limiting reductive acetylation of the lipoyl moiety linked to the dihydrolipoamide acetyltransferase component of the pyruvate dehydrogenase complex. E1 is an α2β2 tetramer (∼154 kDa). Crystals of this recombinant enzyme have been grown in polyethylene glycol 3350 using a vapor-diffusion method at 295 K. The crystals are characterized as orthorhombic, space group P212121, with unit-cell parameters a = 64.2, b = 126.9, c = 190.2 Å. Crystals diffracted to a minimum d spacing of 2.5 Å. The asymmetric unit contains one α2β2 tetrameric E1 assembly; self-rotation function analysis showed a pseudo-twofold symmetry relating the two αβ dimers.
硫胺素噻唑酮焦磷酸与哺乳动物丙酮酸脱氢酶的结合及其对激酶和磷酸酶活性的影响。
DOI：
10.1016/0006-291x(77)90636-2
发表时间：
1977
影响因子：
3.1
作者：
James R. Butler;F. Pettit;P. Davis;Lester J. Reed
通讯作者：
Lester J. Reed
通过磷酸化调节猪心丙酮酸脱氢酶。
DOI：
10.1042/bj1730659
发表时间：
1978
期刊：
The Biochemical journal
影响因子：
--
作者：
P. Sugden;P. J. Randle
通讯作者：
P. J. Randle