Temporal and Spatial Expression of Cyclin H in Rat Spinal Cord Injury
Temporal and Spatial Expression of Cyclin H in Rat Spinal Cord Injury
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DOI：
10.1007/s12017-011-8150-1
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发表时间：
2011-06
影响因子：
3.5
通讯作者：
Gang Wu;Jianhua Cao;Chen Peng;Huiguang Yang;Zhiming Cui;Jian Zhao;Qiyun Wu;Jian-zhong Han
中科院分区：
文献类型：
--
作者：
Gang Wu;Jianhua Cao;Chen Peng;Huiguang Yang;Zhiming Cui;Jian Zhao;Qiyun Wu;Jian-zhong Han
Cyclin H 调节细胞周期转换；它总是与 CDK7 和 MAT1 形成三聚体细胞周期蛋白依赖性蛋白激酶 (CDK) 激活激酶 (CAK) 复合物，磷酸化 CDK2 T 环区域中的苏氨酸残基。然而，cyclin H在中枢神经系统（CNS）损伤中的表达和功能尚不清楚。因此，我们在大鼠脊髓挫伤模型中研究了细胞周期蛋白 H。损伤显着增加了整个胸脊髓的细胞周期蛋白 H 蛋白表达，但没有增加 CDK7。然而，增殖细胞核抗原（PCNA）和细胞标志物的双重免疫荧光染色显示，增殖的小胶质细胞和星形胶质细胞中的cyclin H和CDK2增加，免疫共沉淀研究表明损伤后cyclin H与CDK2的关联明显增强。我们的数据表明，cyclin H 可能在脊髓损伤 (SCI) 中发挥增殖作用。
Cyclin H regulates cell cycle transitions; it always forms trimeric cyclin-dependent protein kinases (CDK)-activating kinase (CAK) complex with CDK7 and MAT1 that phosphorylates a threonine residue in the CDK2 T loop region. However, neither the expression nor function of cyclin H in the central nervous system (CNS) injury is still clear. Therefore, we studied cyclin H in a rat spinal cord contusion model. Injury markedly increased cyclin H protein expression throughout the thoracic spinal cord but did not increase CDK7. However, double immunofluorescent staining for proliferating cell nuclear antigen (PCNA) and cell markers revealed increases of cyclin H and CDK2 in proliferating microglia and astrocytes, and the co-immunoprecipitation studies shown that the associations of cyclin H with CDK2 were enhanced evidently after injury. Our data suggest that cyclin H may play a proliferative role in spinal cord injury (SCI).