EXPRESSION OF THE MAGE GENE FAMILY IN PRIMARY AND METASTATIC HUMAN BREAST-CANCER - IMPLICATIONS FOR TUMOR ANTIGEN-SPECIFIC IMMUNOTHERAPY
EXPRESSION OF THE MAGE GENE FAMILY IN PRIMARY AND METASTATIC HUMAN BREAST-CANCER - IMPLICATIONS FOR TUMOR ANTIGEN-SPECIFIC IMMUNOTHERAPY
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DOI：
10.1002/ijc.2910640313
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发表时间：
1995-06-22
影响因子：
6.4
通讯作者：
BORDIGNON, C
中科院分区：
文献类型：
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作者：
RUSSO, V;TRAVERSARI, C;BORDIGNON, C
一些人类肿瘤表达可用作特异性免疫疗法的靶标的已知抗原。这种治疗策略的功效的绝对要求是原发性和转移性肿瘤的所有细胞充分表达候选抗原。为了检测转移性乳腺癌中肿瘤相关抗原的存在和分布，我们使用PCR分析和溴化乙锭染色检测了28例原发性乳腺癌和相关转移样本中法师家族基因的表达。总体而言，从28名患者中的7名获得的样本显示阳性。然而，3例法师-1和/或法师-3阳性的原发性肿瘤中有2例具有相应的阴性转移病灶。相反，25例MAGE阴性原发肿瘤中有4例出现阳性转移淋巴结。我们的研究结果证实了在体内，在分子水平上，肿瘤抗原的异质性先前观察到的细胞水平的体外分析。我们的数据有力地表明，至少在乳腺癌患者中，需要多种不同的抗原来优化使用法师产品作为靶抗原的免疫治疗方案中的肿瘤细胞识别。(C)1995 Wiley-Liss，Inc.
Some human tumors express known antigens that can be utilized as targets for specific immunotherapy. An absolute requirement for the efficacy of this therapeutic strategy is an adequate expression of the candidate antigen by all cells of the primary and metastatic tumor. To examine the presence and distribution of tumor-associated antigens in metastatic breast cancer, we used PCR analysis and ethidium bromide staining to test the expression of genes of the MAGE family in 28 primary tumors and related metastatic samples. Overall, samples obtained from 7 of 28 patients revealed positive. However, 2 of 3 primary tumors positive for MAGE-1 and/or MAGE-3 had corresponding negative metastatic lesions. On the contrary, 4 of the 25 MAGE-negative primary tumors gave rise to positive metastatic nodes. Our results confirm in vivo, at the molecular level, the tumor-antigen heterogeneity previously observed at the cellular level by in vitro analysis. Our data strongly suggest that, at least in patients with breast cancer, multiple different antigens would be required to optimize the recognition of neoplastic cells in immunotherapeutic protocols using MAGE products as target antigens. (C) 1995 Wiley-Liss, Inc.