Salidroside protects against MPP+-induced apoptosis in PC12 cells by inhibiting the NO pathway
Salidroside protects against MPP+-induced apoptosis in PC12 cells by inhibiting the NO pathway
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DOI：
10.1016/j.brainres.2011.01.015
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发表时间：
2011-03-25
期刊：
影响因子：
2.9
通讯作者：
Chen, Jianzong
中科院分区：
文献类型：
--
作者：
Li, Xiaofeng;Ye, Xiaoli;Chen, Jianzong
氧化应激在帕金森病和其他神经退行性疾病中起着重要作用。红景天苷是一种从红景天中分离得到的苯丙素类糖苷，具有很强的抗氧化特性。在本研究中，我们研究了红景天苷对1-甲基-4-苯基吡啶（MPP+）诱导的PC12细胞凋亡的保护活性。我们发现，孵育的PC12细胞与红景天苷之前MPP+曝光显着减少细胞凋亡和衰减的线粒体膜电位（MMP）的崩溃。红景天苷还能抑制MPP+诱导的NO升高和nNOS、iNOS的过度表达，抑制活性氧（ROS）和细胞内游离Ca~（2+）的积累。我们的研究结果表明，红景天苷对PC12细胞的保护作用介导的，至少部分，通过抑制NO通路。(C)2011 Elsevier B.V.保留所有权利。
Oxidative stress plays an important role in Parkinson's disease and other neurodegenerative disorders. Salidroside, a phenylpropanoid glycoside isolated from Rhodiola rosea L., has potent antioxidant properties. In the present study, we investigated the protective activity of salidroside against 1-methy1-4-phenylpyridinium (MPP+)-induced apoptosis in PC12 cells. We found that incubation of PC12 cells with salidroside prior to MPP+ exposure significantly reduced cell apoptosis and attenuated collapse of the mitochondrial membrane potential (MMP). Furthermore, salidroside inhibited the MPP+-induced nitric oxide (NO) increase and overexpression of nNOS and iNOS and suppressed accumulation of reactive oxygen species (ROS) and intracellular free Ca2+. Our results show that the protective effects of salidroside on PC12 cells are mediated, at least in part, by inhibition of the NO pathway. (C) 2011 Elsevier B.V. All rights reserved.