LbrA, a protein predicted to have a role in vesicle trafficking, is necessary for normal morphogenesis in Polysphondylium pallidum
LbrA, a protein predicted to have a role in vesicle trafficking, is necessary for normal morphogenesis in Polysphondylium pallidum
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DOI：
10.1016/s0378-1119(99)00379-0
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发表时间：
1999-10-18
期刊：
影响因子：
3.5
通讯作者：
Tanaka, Y
中科院分区：
文献类型：
--
作者：
Kawabe, Y;Enomoto, T;Tanaka, Y
苍白多鞭藓的子实体由沿着中轴的轮状分枝组成。在子实体形成过程中，相邻轮的间隔以及轮中分枝的数量和间距受到高度调节。在这项研究中，使用REMI（限制性酶介导的整合）插入诱变方法，我们获得了一个突变株（M2323）具有更长的分支比野生型菌株PN 500。序列分析显示，在载体插入位点附近存在206个氨基酸残基（23 kDa）的ORF。通过同源重组破坏基因IbrA（长分支A）可导致与M2323相同的表型。在亲本菌株中，IbrA转录物在早期聚集阶段最大程度地表达，但在REMI突变体中检测不到。同源性搜索表明，LbrA是p24家族蛋白的成员，其已被提出作为货物蛋白的受体，所述货物蛋白由COP I-（外壳蛋白I）和/或COP II-包被的囊泡在内质网和高尔基复合体之间转运。据我们所知，这是第一篇表明p24家族成员与形态发生有关的论文。(C)1999 Elsevier Science B. V.保留所有权利。
The fruiting body of Polysphondylium pallidum is composed of whorls of branches along the axis of a central stalk. In the course of fruiting body formation, the interval between neighboring whorls, and the number and the spacing of branches in a whorl are highly regulated. In this study, using the REMI (restriction-enzyme-mediated integration) insertional mutagenesis method, we obtained a mutant (strain M2323) with longer branches than those of the wild-type strain PN500. The sequence analyses revealed the presence of an ORF of 206 aa residues (23 kDa) near the vector insertion site. Disruption of the gene, IbrA (long branch A), by homologous recombination causes the same phenotype as that of M2323. A IbrA transcript is expressed maximally at the early aggregation stage in the parental strain, but is not detectable in the REMI mutant. A homology search showed that LbrA is a member of the p24 family proteins, which have been proposed to function as receptors for cargo proteins that are transported by COP I- (coat protein I) and/or COP II-coated vesicles between the endoplasmic reticulum and the Golgi complex. As far as we know, this is the first paper to show that a p24 family member is implicated in morphogenesis. (C) 1999 Elsevier Science B.V. All rights reserved.