Application of the polymerase chain reaction for the diagnosis of fowl poxvirus infection
Application of the polymerase chain reaction for the diagnosis of fowl poxvirus infection
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DOI：
10.1016/s0166-0934(96)02119-2
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发表时间：
1997-01-01
影响因子：
3.1
通讯作者：
Lee, KH
中科院分区：
文献类型：
--
作者：
Lee, LH;Lee, KH
聚合酶链式反应 (PCR) 用于扩增禽痘病毒 (FPV) 基因组的 578 bp 片段，并使用一组引物构建了编码 4b 核心蛋白的基因内的区域。在六种 FPV 菌株中检测到扩增产物，而未从未感染的细胞培养物、皮肤组织或四种不相关的禽类病原体中检测到扩增产物。使用来自 FPV 感染的细胞培养物的核酸测试 PCR 的敏感性。溴化乙锭染色凝胶中的检测限为 10(-1) TCID50。此外，该检测系统还用于检测商业饲养鸡的皮肤和呼吸道拭子组织样本中的 FPV。通过使用简单、快速的程序进一步确定来自组织样本制备物的扩增产物的身份，其中使用内部嵌套的末端标记探针。版权所有 (C) 1997 Elsevier Science B.V.
The polymerase chain reaction (PCR) was used to amplify a 578-bp fragment of the fowl poxvirus (FPV) genome and with a set of primers framed a region within the gene coding for 4b core protein. An amplified product was detected with six strains of FPV, whereas none was obtained from uninfected cell cultures, skin tissue or four unrelated avian pathogens. The sensitivity of PCR was tested with nucleic acids from the FPV-infected cell cultures. The detection limit was 10(-1) TCID50 in an ethidium bromide-stained gel. In addition, this assay system was used to detect FPV in tissue specimens of skin and respiratory swabs collected from commercially reared chickens. The identity of the amplification products from the tissue specimen preparations was determined further by using a simple, rapid procedure in which an internally nested, end-labeled probe was used. Copyright (C) 1997 Elsevier Science B.V.