Electrochemical evaluation of alternating duplex-triplex conversion effect on the anthraquinone-photoinjected hole transport through DNA duplex immobilized on a gold electrode.
Electrochemical evaluation of alternating duplex-triplex conversion effect on the anthraquinone-photoinjected hole transport through DNA duplex immobilized on a gold electrode.
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DOI：
10.1021/ja0568244
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发表时间：
2006-01
影响因子：
15
通讯作者：
K. Tanabe;Haruka Iida;Ken-ichi Haruna;T. Kamei;A. Okamoto;S. Nishimoto
中科院分区：
文献类型：
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作者：
K. Tanabe;Haruka Iida;Ken-ichi Haruna;T. Kamei;A. Okamoto;S. Nishimoto
采用安培法表征蒽醌（AQ）-光注入空穴传输通过20聚体寡脱氧核苷酸（ODN）双链体，固定在金电极的表面上，其三链体的形式转换与几个第三寡嘧啶（OPD）短链协会。虽然在365 nm照射连接到DNA双链体末端的AQ光敏剂时观察到阴极光电流，但通过与给定的第三OPD短链形成双链体的三链体，确定发生电流密度的显著降低。通过DNA双链体的光电流表现出可逆的下降-上升响应，伴随着OPD短链的交替缔合-解离循环，如通过温度变化调节的DNA三链体的相应解链温度周围。开关光辐射和热交替双链-三链转换都可以提供调控DNA空穴输运的工具。
Amperometry was employed to characterize the anthraquinone (AQ)-photoinjected hole transport through a 20-mer oligodeoxynucleotide (ODN) duplex, as immobilized on the surface of a gold electrode, and its triplex forms converted by association with several third oligopyrimidine (OPD) short strands. While the cathodic photocurrent was observed upon irradiation at 365 nm of the AQ photosensitizer linked to the end of DNA duplex, a marked lowering of the current density was identified to occur by the triplex formation of a duplex with a given third OPD short strand. The photocurrent through the DNA duplex showed a reversible fall-rise response concomitant with alternating association-dissociation cycle of the OPD short-strand, as regulated by temperature change around the corresponding melting temperature of the DNA triplex. Both the switched photoirradiation and the thermally alternating duplex-triplex conversion could provide tools of regulating the DNA hole transport.