Detection of tumor necrosis factor α in normal and inflamed human dental pulps
Detection of tumor necrosis factor α in normal and inflamed human dental pulps
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DOI：
10.1016/s0188-4409(02)00396-x
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发表时间：
2002-09-01
影响因子：
7.7
通讯作者：
Simunovic-Soskic, M
中科院分区：
文献类型：
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作者：
Pezelj-Ribaric, S;Anic, I;Simunovic-Soskic, M
背景本研究的目的是确定肿瘤坏死因子-α（TNF-α）的浓度在正常，疼痛，和无症状的人牙髓。从三组牙齿中获得牙髓，包括健康牙齿、具有龋齿和/或大修复体的无症状牙齿以及临床诊断为不可逆牙髓炎的有症状牙齿。收集牙髓组织，制备并通过ELISA技术分析TNF-α的浓度。非参数Kruskal-Wallis检验显示正常样本中TNF-α浓度之间存在显著差异（64.01 +/- 53.12 pg/g）和不可逆的症状性牙髓组织（1，962.99 +/-1，288.75 pg/g），介于不可逆的有症状和无症状之间（1，120.09 +/- 649.72 pg/g），正常和不可逆的无症状牙髓组织之间（p = 0.000）。TNF-α可作为判断不可逆性牙髓炎牙髓炎症程度的客观指标。(C)2002年IMSS。出版社：Elsevier Science Inc.
Background. The aim of this study was to determine concentrations of tumor necrosis factor-alpha (TNF-alpha) in normal, painful, and asymptomatic human dental pulps.Methods. Pulps were obtained from three groups of teeth, including healthy teeth, asymptomatic teeth with caries and/or large restoration, and symptomatic teeth with clinical diagnosis of irreversible pulpitis. Pulpal tissues were collected, prepared, and analyzed for TNF-alpha concentration by ELISA technique.Results. Nonparametric Kruskal-Wallis test revealed significant differences between TNF-alpha concentration in normal samples (64.01 +/- 53.12 pg/g) and irreversible symptomatic pulpal tissue (1,962.99 +/- 1,288.75 pg/g), between irreversible symptomatic and asymptomatic (1, 120.09 +/- 649.72 pg/g), and between normal and irreversible asymptomatic pulpal tissue (p = 0.000).Conclusions. TNF-alpha may be an objective marker for determining extent of pulpal inflammation associated with irreversible pulpitis. (C) 2002 IMSS. Published by Elsevier Science Inc.