Cell Wall Extensibility and Effect of Cell-Wall-Loosening Proteins during Rose Flower Opening
Cell Wall Extensibility and Effect of Cell-Wall-Loosening Proteins during Rose Flower Opening
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DOI：
10.2503/jjshs1.78.242
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发表时间：
2009-04
影响因子：
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通讯作者：
K. Yamada;R. Takahashi;C. Fujitani;Keiko Mishima;Masato Yoshida;D. Joyce;S. Yamaki
中科院分区：
文献类型：
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作者：
K. Yamada;R. Takahashi;C. Fujitani;Keiko Mishima;Masato Yoshida;D. Joyce;S. Yamaki
由于花的开放涉及花瓣细胞的扩张，因此对Rosa hybrida L. ' FEbesa ' （syn. ' Pretty Woman '）花瓣的扩张生长机制进行了研究。用蠕变伸展分析法分析花瓣细胞壁的伸展性。花瓣细胞壁在完全分离的花萼发育的第4期变得松散。此外，从IV期玫瑰花瓣中提取的细胞壁蛋白被认为对III期花瓣的细胞壁松动有积极作用。扩张蛋白和木葡聚糖内转糖基化酶/水解酶（XTH）蛋白位于外质体上，可能与植物细胞壁松动有关。从玫瑰花瓣中提取了这些细胞壁蛋白的转录本，以阐明它们在玫瑰开花中的潜在作用。在花瓣发育过程中，扩展蛋白和XTH转录物水平发生了显著变化。其中，RhEXPA1和RhXTH1转录本水平在花瓣生长过程中显著升高，与花瓣不同部位的典型生长有关。基于以上研究结果，我们认为RhEXPA1和RhXTH1是参与玫瑰花瓣膨胀生长的主要同源基因。
As flower opening involves the expansion of petal cells, the mechanisms of expansion growth were investigated in Rosa hybrida L. ‘FEbesa’ (syn. ‘Pretty Woman’) petals. Petal cell-wall extensibility was analyzed by creep extension analysis. Walls of the petal cells became loose at developing stage IV of completely separated sepals on an opening flower. Furthermore, cell-wall proteins extracted from rose petals at stage IV were suggested to have a positive effect on cell-wall loosening of stage III petals. Expansin and xyloglucan endotransglycosylase/hydrolase (XTH) proteins, which are located on apoplasts, are probably involved in cell-wall loosening in plants. Transcripts of these cell-wall proteins were extracted from rose petals with a view to elucidating their potential role in rose flower opening. Expansin and XTH transcript levels changed markedly during petal development. In particular, transcript levels of RhEXPA1 and RhXTH1 increased markedly during petal growth and were relevant to the typical growth in different part of the petals. Based on the present findings, we suggest that RhEXPA1 and RhXTH1 are the major paralogs involved in expansion growth of rose petals.